DOI: 10.1073/pnas.0809406106. Heme uptake across the outer membrane as revealed by crystal structures of the receptor

Heme uptake across the outer membrane as revealed by crystal structures of the receptor–hemophore complex

10.1073/pnas.0809406106

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Gram-negative bacteria use specific heme uptake systems, relying on outer membrane receptors and excreted heme-binding proteins (hemophores) to scavenge and actively transport heme. To unravel the unknown molecular details involved, we present 3 structures of the Serratia marcescens receptor HasR in complex with its hemophore HasA. The transfer of heme over a distance of 9 Å from its high-affinity site in HasA into a site of lower affinity in HasR is coupled with the exergonic complex formation of the 2 proteins. Upon docking to the receptor, 1 of the 2 axial heme coordinations of the hemophore is initially broken, but the position and orientation of the heme is preserved. Subsequently, steric displacement of heme by a receptor residue ruptures the other axial coordination, leading to heme transfer into the receptor.

Bibliography

Krieg, S., HuchÃ©, F., Diederichs, K., Izadi-Pruneyre, N., Lecroisey, A., Wandersman, C., Delepelaire, P., & Welte, W. (2009). Heme uptake across the outer membrane as revealed by crystal structures of the receptorâhemophore complex. Proceedings of the National Academy of Sciences, 106(4), 1045â1050.

Authors 8

Stefanie Krieg (first)
Frédéric Huché (additional)
Kay Diederichs (additional)
Nadia Izadi-Pruneyre (additional)
Anne Lecroisey (additional)
Cécile Wandersman (additional)
Philippe Delepelaire (additional)
Wolfram Welte (additional)

References 41 Referenced 143

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Dates

Type	When
Created	16 years, 7 months ago (Jan. 14, 2009, 10:42 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 6:02 p.m.)
Indexed	1 week, 5 days ago (Aug. 20, 2025, 9:22 a.m.)
Issued	16 years, 7 months ago (Jan. 27, 2009)
Published	16 years, 7 months ago (Jan. 27, 2009)
Published Online	16 years, 7 months ago (Jan. 27, 2009)
Published Print	16 years, 7 months ago (Jan. 27, 2009)

Funders 0

None

BibTeX

@article{Krieg_2009, title={Heme uptake across the outer membrane as revealed by crystal structures of the receptor–hemophore complex}, volume={106}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0809406106}, DOI={10.1073/pnas.0809406106}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Krieg, Stefanie and Huché, Frédéric and Diederichs, Kay and Izadi-Pruneyre, Nadia and Lecroisey, Anne and Wandersman, Cécile and Delepelaire, Philippe and Welte, Wolfram}, year={2009}, month=jan, pages={1045–1050} }

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