DOI: 10.1073/pnas.0807683105. Domain compliance and elastic power transmission in rotary F <sub>O</sub> F <sub>1</sub> -ATPase

Domain compliance and elastic power transmission in rotary F O F 1 -ATPase

10.1073/pnas.0807683105

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The 2 nanomotors of rotary ATP synthase, ionmotive F O and chemically active F 1 , are mechanically coupled by a central rotor and an eccentric bearing. Both motors rotate, with 3 steps in F 1 and 10–15 in F O . Simulation by statistical mechanics has revealed that an elastic power transmission is required for a high rate of coupled turnover. Here, we investigate the distribution in the F O F 1 structure of compliant and stiff domains. The compliance of certain domains was restricted by engineered disulfide bridges between rotor and stator, and the torsional stiffness (κ) of unrestricted domains was determined by analyzing their thermal rotary fluctuations. A fluorescent magnetic bead was attached to single molecules of F 1 and a fluorescent actin filament to F O F 1 , respectively. They served to probe first the functional rotation and, after formation of the given disulfide bridge, the stochastic rotational motion. Most parts of the enzyme, in particular the central shaft in F 1 , and the long eccentric bearing were rather stiff (torsional stiffness κ > 750 pNnm). One domain of the rotor, namely where the globular portions of subunits γ and ε of F 1 contact the c-ring of F O , was more compliant (κ ≅ 68 pNnm). This elastic buffer smoothes the cooperation of the 2 stepping motors. It is located were needed, between the 2 sites where the power strokes in F O and F 1 are generated and consumed.

Bibliography

Sielaff, H., Rennekamp, H., WÃ¤chter, A., Xie, H., Hilbers, F., Feldbauer, K., Dunn, S. D., Engelbrecht, S., & Junge, W. (2008). Domain compliance and elastic power transmission in rotary F O F 1 -ATPase. Proceedings of the National Academy of Sciences, 105(46), 17760â17765.

Authors 9

Hendrik Sielaff (first)
Henning Rennekamp (additional)
André Wächter (additional)
Hao Xie (additional)
Florian Hilbers (additional)
Katrin Feldbauer (additional)
Stanley D. Dunn (additional)
Siegfried Engelbrecht (additional)
Wolfgang Junge (additional)

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Dates

Type	When
Created	16 years, 9 months ago (Nov. 10, 2008, 9:25 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 5:55 p.m.)
Indexed	3 weeks, 2 days ago (Aug. 6, 2025, 9:06 a.m.)
Issued	16 years, 9 months ago (Nov. 18, 2008)
Published	16 years, 9 months ago (Nov. 18, 2008)
Published Online	16 years, 9 months ago (Nov. 18, 2008)
Published Print	16 years, 9 months ago (Nov. 18, 2008)

Funders 0

None

BibTeX

@article{Sielaff_2008, title={Domain compliance and elastic power transmission in rotary F O F 1 -ATPase}, volume={105}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0807683105}, DOI={10.1073/pnas.0807683105}, number={46}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sielaff, Hendrik and Rennekamp, Henning and Wächter, André and Xie, Hao and Hilbers, Florian and Feldbauer, Katrin and Dunn, Stanley D. and Engelbrecht, Siegfried and Junge, Wolfgang}, year={2008}, month=nov, pages={17760–17765} }

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