DOI: 10.1073/pnas.080585897. The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p

The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p

10.1073/pnas.080585897

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In Saccharomyces cerevisiae , mRNA encoding the cell-fate determinant Ash1p is localized to the distal tip of daughter cells. Five SHE genes are required for proper Ash1 mRNA localization, one of which encodes the myosin Myo4p. We show that three of the five She proteins, She2p, She3p, and Myo4p, colocalize with Ash1 mRNA in vivo and coimmunoprecipitate with Ash1 mRNA from cell extracts. We also find that She3p binds to Myo4p in the absence of RNA and She2p is required for binding She3p-Myo4p to Ash1 mRNA. These results suggest that She3p acts as an adapter protein that docks the myosin motor onto an Ash1–She2p ribonucleoprotein complex.

Bibliography

Takizawa, P. A., & Vale, R. D. (2000). The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p. Proceedings of the National Academy of Sciences, 97(10), 5273â5278.

Authors 2

Peter A. Takizawa (first)
Ronald D. Vale (additional)

References 22 Referenced 170

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:32 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 5:36 p.m.)
Indexed	4 weeks, 1 day ago (July 26, 2025, 4:59 a.m.)
Issued	25 years, 3 months ago (May 2, 2000)
Published	25 years, 3 months ago (May 2, 2000)
Published Online	25 years, 3 months ago (May 2, 2000)
Published Print	25 years, 3 months ago (May 9, 2000)

Funders 0

None

BibTeX

@article{Takizawa_2000, title={The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.080585897}, DOI={10.1073/pnas.080585897}, number={10}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Takizawa, Peter A. and Vale, Ronald D.}, year={2000}, month=may, pages={5273–5278} }

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