DOI: 10.1073/pnas.080536597. SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage

SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage

10.1073/pnas.080536597

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Ubiquitin/26S proteasome-dependent degradation of topoisomerase I (TOP1) has been suggested to be a unique repair response to TOP1-mediated DNA damage. In the current study, we show that treatment of mammalian cells or yeast cells expressing human DNA TOP1 with camptothecin (CPT) induces covalent modification of the TOP1 by SUMO-1/Smt3p, a ubiquitin-like protein. This conclusion is based on the following observations: (i) Mammalian DNA TOP1 conjugates induced by CPT were cross-reactive with SUMO-1/Smt3p-specific antibodies both in yeast expressing human DNA TOP1 as well as mammalian cells. (ii) The formation of TOP1 conjugates was shown to be dependent on UBC9, the E2 enzyme for SUMO-1/Smt3p. (iii) TOP1 physically interacts with UBC9. (iv) Ubc9 mutant yeast cells expressing human DNA TOP1 was hypersensitive to CPT, suggesting that UBC9/SUMO-1 may be involved in the repair of TOP1-mediated DNA damage.

Bibliography

Mao, Y., Sun, M., Desai, S. D., & Liu, L. F. (2000). SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage. Proceedings of the National Academy of Sciences, 97(8), 4046â4051.

Authors 4

Yong Mao (first)
Mei Sun (additional)
Shyamal D. Desai (additional)
Leroy F. Liu (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:38 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:19 a.m.)
Indexed	1 month, 1 week ago (July 16, 2025, 8:47 a.m.)
Issued	25 years, 4 months ago (April 4, 2000)
Published	25 years, 4 months ago (April 4, 2000)
Published Online	25 years, 4 months ago (April 4, 2000)
Published Print	25 years, 4 months ago (April 11, 2000)

Funders 0

None

BibTeX

@article{Mao_2000, title={SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.080536597}, DOI={10.1073/pnas.080536597}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Mao, Yong and Sun, Mei and Desai, Shyamal D. and Liu, Leroy F.}, year={2000}, month=apr, pages={4046–4051} }

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      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2000-04-04",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}