DOI: 10.1073/pnas.0802301105. Alternative translation of osteopontin generates intracellular and secreted isoforms that mediate distinct biological activities in dendritic cells

Alternative translation of osteopontin generates intracellular and secreted isoforms that mediate distinct biological activities in dendritic cells

10.1073/pnas.0802301105

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Osteopontin (Opn) contributes to diverse biological processes that include immune responses, vascularization, and bone formation. Until recently, studies describing the activities of Opn have focused on the cytokine-like properties of the secreted protein. Here, we show that alternative translation of a single Opn mRNA species generates a secreted and intracellular isoform. Utilization of a 5′ canonical translation start site generates a protein that includes an N-terminal signal sequence allowing targeting to secretory vesicles and cytokine secretion, whereas usage of a downstream start site generates a shortened protein that lacks the N-terminal signal sequence and localizes mainly to cytoplasm. The coordinated action of these Opn gene products regulates the functional phenotype of subsets of dendritic cells.

Bibliography

Shinohara, M. L., Kim, H.-J., Kim, J.-H., Garcia, V. A., & Cantor, H. (2008). Alternative translation of osteopontin generates intracellular and secreted isoforms that mediate distinct biological activities in dendritic cells. Proceedings of the National Academy of Sciences, 105(20), 7235â7239.

Authors 5

Mari L. Shinohara (first)
Hye-Jung Kim (additional)
June-Ho Kim (additional)
Virgilio A. Garcia (additional)
Harvey Cantor (additional)

References 31 Referenced 171

Dates

Type	When
Created	17 years, 3 months ago (May 14, 2008, 9:10 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 4:12 p.m.)
Indexed	1 week, 6 days ago (Aug. 12, 2025, 6:10 p.m.)
Issued	17 years, 3 months ago (May 20, 2008)
Published	17 years, 3 months ago (May 20, 2008)
Published Online	17 years, 3 months ago (May 20, 2008)
Published Print	17 years, 3 months ago (May 20, 2008)

Funders 0

None

BibTeX

@article{Shinohara_2008, title={Alternative translation of osteopontin generates intracellular and secreted isoforms that mediate distinct biological activities in dendritic cells}, volume={105}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0802301105}, DOI={10.1073/pnas.0802301105}, number={20}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Shinohara, Mari L. and Kim, Hye-Jung and Kim, June-Ho and Garcia, Virgilio A. and Cantor, Harvey}, year={2008}, month=may, pages={7235–7239} }

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