DOI: 10.1073/pnas.0802177105. Unraveling the molecular basis of subunit specificity in P pilus assembly by mass spectrometry

Unraveling the molecular basis of subunit specificity in P pilus assembly by mass spectrometry

10.1073/pnas.0802177105

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

P pili are multisubunit fibers essential for the attachment of uropathogenic Escherichia coli to the kidney. These fibers are formed by the noncovalent assembly of six different homologous subunit types in an array that is strictly defined in terms of both the number and order of each subunit type. Assembly occurs through a mechanism termed “donor-strand exchange (DSE)” in which an N-terminal extension (Nte) of one subunit donates a β-strand to an adjacent subunit, completing its Ig fold. Despite structural determination of the different subunits, the mechanism determining specificity of subunit ordering in pilus assembly remained unclear. Here, we have used noncovalent mass spectrometry to monitor DSE between all 30 possible pairs of P pilus subunits and their Ntes. We demonstrate a striking correlation between the natural order of subunits in pili and their ability to undergo DSE in vitro . The results reveal insights into the molecular mechanism by which subunit ordering during the assembly of this complex is achieved.

Bibliography

Rose, R. J., Verger, D., Daviter, T., Remaut, H., Paci, E., Waksman, G., Ashcroft, A. E., & Radford, S. E. (2008). Unraveling the molecular basis of subunit specificity in P pilus assembly by mass spectrometry. Proceedings of the National Academy of Sciences, 105(35), 12873â12878.

Authors 8

Rebecca J. Rose (first)
Denis Verger (additional)
Tina Daviter (additional)
Han Remaut (additional)
Emanuele Paci (additional)
Gabriel Waksman (additional)
Alison E. Ashcroft (additional)
Sheena E. Radford (additional)

References 27 Referenced 56

Dates

Type	When
Created	17 years ago (Aug. 26, 2008, 8:45 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 4 p.m.)
Indexed	2 months, 4 weeks ago (May 29, 2025, 1:23 p.m.)
Issued	16 years, 11 months ago (Sept. 2, 2008)
Published	16 years, 11 months ago (Sept. 2, 2008)
Published Online	16 years, 11 months ago (Sept. 2, 2008)
Published Print	16 years, 11 months ago (Sept. 2, 2008)

Funders 0

None

BibTeX

@article{Rose_2008, title={Unraveling the molecular basis of subunit specificity in P pilus assembly by mass spectrometry}, volume={105}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0802177105}, DOI={10.1073/pnas.0802177105}, number={35}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Rose, Rebecca J. and Verger, Denis and Daviter, Tina and Remaut, Han and Paci, Emanuele and Waksman, Gabriel and Ashcroft, Alison E. and Radford, Sheena E.}, year={2008}, month=sep, pages={12873–12878} }

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