DOI: 10.1073/pnas.080077197. Association of <i>Escherichia coli</i> ribosomes with the inner membrane requires the signal recognition particle receptor but is independent of the signal recognition particle

Association of Escherichia coli ribosomes with the inner membrane requires the signal recognition particle receptor but is independent of the signal recognition particle

10.1073/pnas.080077197

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In mammalian cells, as well as Escherichia coli , ribosomes translating membrane proteins interact cotranslationally with translocons in the membrane, and this interaction is essential for proper insertion of nascent polypeptides into the membrane. Both the signal recognition particle (SRP) and its receptor (SR) are required for functional association of ribosomes translating integral membrane proteins with the translocon. Herein, we confirm that membrane targeting of E. coli ribosomes requires the prokaryotic SRα homolog FtsY in vivo . Surprisingly, however, depletion of the E. coli SRP54 homolog (Ffh) has no significant effect on binding of ribosomes to the membrane, although Ffh depletion is detrimental to growth. These and other observations suggest that, in E. coli , SRP may operate downstream of SR-mediated targeting of ribosomes to the plasma membrane.

Bibliography

Herskovits, A. A., & Bibi, E. (2000). Association of Escherichia coli ribosomes with the inner membrane requires the signal recognition particle receptor but is independent of the signal recognition particle. Proceedings of the National Academy of Sciences, 97(9), 4621â4626.

Authors 2

Anat A. Herskovits (first)
Eitan Bibi (additional)

References 29 Referenced 72

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 4:11 p.m.)
Indexed	1 year ago (Aug. 11, 2024, 3:11 p.m.)
Issued	25 years, 4 months ago (April 18, 2000)
Published	25 years, 4 months ago (April 18, 2000)
Published Online	25 years, 4 months ago (April 18, 2000)
Published Print	25 years, 4 months ago (April 25, 2000)

Funders 0

None

BibTeX

@article{Herskovits_2000, title={Association of Escherichia coli ribosomes with the inner membrane requires the signal recognition particle receptor but is independent of the signal recognition particle}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.080077197}, DOI={10.1073/pnas.080077197}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Herskovits, Anat A. and Bibi, Eitan}, year={2000}, month=apr, pages={4621–4626} }

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