DOI: 10.1073/pnas.080064697. The three-dimensional structure of halorhodopsin to 5 Å by electron crystallography: A new unbending procedure for two-dimensional crystals by using a global reference structure

The three-dimensional structure of halorhodopsin to 5 Å by electron crystallography: A new unbending procedure for two-dimensional crystals by using a global reference structure

10.1073/pnas.080064697

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Electron microscopy does not, in principle, require highly ordered crystals to determine a high-resolution structure. Nevertheless, crystals of any type help to constrain the molecules into a more limited range of orientations and positions, from which it is easier to carry out structure determination. We describe an improved procedure for determination of crystalline disorder, which we have applied to poorly ordered two-dimensional crystals of the chloride pump halorhodopsin from Halobacterium salinarum . The new image analysis procedure involves the use of a reference projection calculated from a global three-dimensional map to carry out the initial cross-correlation analysis. Coupled with a greater number of images taken with field emission gun microscopes, this has allowed us to calculate a three-dimensional structure for halorhodopsin, in which the seven transmembrane helices and certain molecular features, such as the β-ionone ring of retinal, are now resolved.

Bibliography

Kunji, E. R. S., von Gronau, S., Oesterhelt, D., & Henderson, R. (2000). The three-dimensional structure of halorhodopsin to 5 â« by electron crystallography: A new unbending procedure for two-dimensional crystals by using a global reference structure. Proceedings of the National Academy of Sciences, 97(9), 4637â4642.

Authors 4

Edmund R. S. Kunji (first)
Susanne von Gronau (additional)
Dieter Oesterhelt (additional)
Richard Henderson (additional)

References 24 Referenced 40

10.1093/oxfordjournals.jbchem.a022332
10.1110/ps.8.2.447
10.1046/j.1365-2958.1998.00859.x
10.1073/pnas.88.11.4751
10.1016/S0021-9258(18)41872-8
10.1126/science.7604281
10.1006/jmbi.1997.1204
10.1073/pnas.90.2.639
10.1021/bi9601159
10.1016/S0959-440X(99)80065-7
10.1006/jmbi.1993.1629
W A Havelka, R Henderson, D Oesterhelt J Mol Biol 247, 726–738 (1995). / J Mol Biol by Havelka W A (1995)
10.1111/j.1365-2958.1993.tb01153.x
10.1016/0304-3991(87)90147-1
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10.1006/jmbi.1995.0627

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:38 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 4:11 p.m.)
Indexed	4 months, 1 week ago (April 12, 2025, 1:07 a.m.)
Issued	25 years, 4 months ago (April 11, 2000)
Published	25 years, 4 months ago (April 11, 2000)
Published Online	25 years, 4 months ago (April 11, 2000)
Published Print	25 years, 3 months ago (April 25, 2000)

Funders 0

None

BibTeX

@article{Kunji_2000, title={The three-dimensional structure of halorhodopsin to 5 Å by electron crystallography: A new unbending procedure for two-dimensional crystals by using a global reference structure}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.080064697}, DOI={10.1073/pnas.080064697}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kunji, Edmund R. S. and von Gronau, Susanne and Oesterhelt, Dieter and Henderson, Richard}, year={2000}, month=apr, pages={4637–4642} }

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