DOI: 10.1073/pnas.080063997. RNA polymerase I transcription factor Rrn3 is functionally conserved between yeast and human

RNA polymerase I transcription factor Rrn3 is functionally conserved between yeast and human

10.1073/pnas.080063997

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have cloned a human cDNA that is related to the RNA polymerase I transcription factor Rrn3 of Saccharomyces cerevisiae . The recombinant human protein displays both sequence similarity and immunological crossreactivity to yeast Rrn3 and is capable of rescuing a yeast strain carrying a disruption of the RRN3 gene in vivo . Point mutation of an amino acid that is conserved between the yeast and human proteins compromises the function of each factor, confirming that the observed sequence similarity is functionally significant. Rrn3 is the first RNA polymerase I-specific transcription factor shown to be functionally conserved between yeast and mammals, suggesting that at least one mechanism that regulates ribosomal RNA synthesis is conserved among eukaryotes.

Bibliography

Moorefield, B., Greene, E. A., & Reeder, R. H. (2000). RNA polymerase I transcription factor Rrn3 is functionally conserved between yeast and human. Proceedings of the National Academy of Sciences, 97(9), 4724â4729.

Authors 3

Beth Moorefield (first)
Elizabeth A. Greene (additional)
Ronald H. Reeder (additional)

References 27 Referenced 95

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:41 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 4:11 p.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:52 a.m.)
Issued	25 years, 4 months ago (April 11, 2000)
Published	25 years, 4 months ago (April 11, 2000)
Published Online	25 years, 4 months ago (April 11, 2000)
Published Print	25 years, 4 months ago (April 25, 2000)

Funders 0

None

BibTeX

@article{Moorefield_2000, title={RNA polymerase I transcription factor Rrn3 is functionally conserved between yeast and human}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.080063997}, DOI={10.1073/pnas.080063997}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Moorefield, Beth and Greene, Elizabeth A. and Reeder, Ronald H.}, year={2000}, month=apr, pages={4724–4729} }

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