DOI: 10.1073/pnas.0737415100. Ring-like pore structures of SecA: Implication for bacterial protein-conducting channels

Ring-like pore structures of SecA: Implication for bacterial protein-conducting channels

10.1073/pnas.0737415100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

SecA, an essential component of the general protein secretion pathway of bacteria, is present in Escherichia coli as soluble and membrane-integral forms. Here we show by electron microscopy that SecA assumes two characteristic forms in the presence of phospholipid monolayers: dumbbell-shaped elongated structures and ring-like pore structures. The ring-like pore structures with diameters of 8 nm and holes of 2 nm are found only in the presence of anionic phospholipids. These ring-like pore structures with larger 3- to 6-nm holes (without staining) were also observed by atomic force microscopic examination. They do not form in solution or in the presence of uncharged phosphatidylcholine. These ring-like phospholipid-induced pore-structures may form the core of bacterial protein-conducting channels through bacterial membranes.

Bibliography

Wang, H.-W., Chen, Y., Yang, H., Chen, X., Duan, M.-X., Tai, P. C., & Sui, S.-F. (2003). Ring-like pore structures of SecA: Implication for bacterial protein-conducting channels. Proceedings of the National Academy of Sciences, 100(7), 4221â4226.

Authors 7

Hong-Wei Wang (first)
Yong Chen (additional)
Hsiuchin Yang (additional)
Xianchuan Chen (additional)
Ming-Xing Duan (additional)
Phang C. Tai (additional)
Sen-Fang Sui (additional)

References 74 Referenced 59

Dates

Type	When
Created	22 years, 5 months ago (April 1, 2003, 2:25 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 4:49 p.m.)
Indexed	1 year ago (Aug. 11, 2024, 7:49 a.m.)
Issued	22 years, 5 months ago (March 17, 2003)
Published	22 years, 5 months ago (March 17, 2003)
Published Online	22 years, 5 months ago (March 17, 2003)
Published Print	22 years, 5 months ago (April 1, 2003)

Funders 0

None

BibTeX

@article{Wang_2003, title={Ring-like pore structures of SecA: Implication for bacterial protein-conducting channels}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0737415100}, DOI={10.1073/pnas.0737415100}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wang, Hong-Wei and Chen, Yong and Yang, Hsiuchin and Chen, Xianchuan and Duan, Ming-Xing and Tai, Phang C. and Sui, Sen-Fang}, year={2003}, month=mar, pages={4221–4226} }

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  "abstract": "<jats:p>\n            SecA, an essential component of the general protein secretion pathway of bacteria, is present in\n            <jats:italic>Escherichia coli</jats:italic>\n            as soluble and membrane-integral forms. Here we show by electron microscopy that SecA assumes two characteristic forms in the presence of phospholipid monolayers: dumbbell-shaped elongated structures and ring-like pore structures. The ring-like pore structures with diameters of 8 nm and holes of 2 nm are found only in the presence of anionic phospholipids. These ring-like pore structures with larger 3- to 6-nm holes (without staining) were also observed by atomic force microscopic examination. They do not form in solution or in the presence of uncharged phosphatidylcholine. These ring-like phospholipid-induced pore-structures may form the core of bacterial protein-conducting channels through bacterial membranes.\n          </jats:p>",
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