DOI: 10.1073/pnas.072385099. Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2

Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2

10.1073/pnas.072385099

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Proteolysis triggered by the anaphase-promoting complex (APC) is needed for sister chromatid separation and the exit from mitosis. APC is a ubiquitin ligase whose activity is tightly controlled during the cell cycle. To identify factors involved in the regulation of APC-mediated proteolysis, a Saccharomyces cerevisiae GAL-cDNA library was screened for genes whose overexpression prevented degradation of an APC target protein, the mitotic cyclin Clb2. Genes encoding G 1 , S, and mitotic cyclins were identified, consistent with previous data showing that the cyclin-dependent kinase Cdk1 associated with different cyclins is a key factor for inhibiting APC Cdh1 activity from late-G 1 phase until mitosis. In addition, the meiosis-specific protein kinase Ime2 was identified as a negative regulator of APC-mediated proteolysis. Ectopic expression of IME2 in G 1 arrested cells inhibited the degradation of mitotic cyclins and of other APC substrates. IME2 expression resulted in the phosphorylation of Cdh1 in G 1 cells, indicating that Ime2 and Cdk1 regulate APC Cdh1 in a similar manner. The expression of IME2 in cycling cells inhibited bud formation and caused cells to arrest in mitosis. We show further that Ime2 itself is an unstable protein whose proteolysis occurs independently of the APC and SCF (Skp1/Cdc53/F-box) ubiquitin ligases. Our findings suggest that Ime2 represents an unstable, meiosis-specific regulator of APC Cdh1 .

Bibliography

Bolte, M., Steigemann, P., Braus, G. H., & Irniger, S. (2002). Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2. Proceedings of the National Academy of Sciences, 99(7), 4385â4390.

Authors 4

Melanie Bolte (first)
Patrick Steigemann (additional)
Gerhard H. Braus (additional)
Stefan Irniger (additional)

References 44 Referenced 34

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:37 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 4:53 p.m.)
Indexed	1 year, 3 months ago (May 11, 2024, 3:45 a.m.)
Issued	23 years, 5 months ago (March 26, 2002)
Published	23 years, 5 months ago (March 26, 2002)
Published Online	23 years, 5 months ago (March 26, 2002)
Published Print	23 years, 4 months ago (April 2, 2002)

Funders 0

None

BibTeX

@article{Bolte_2002, title={Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.072385099}, DOI={10.1073/pnas.072385099}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Bolte, Melanie and Steigemann, Patrick and Braus, Gerhard H. and Irniger, Stefan}, year={2002}, month=mar, pages={4385–4390} }

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