DOI: 10.1073/pnas.0712247105. <i>In vitro</i> self-assembly of tailorable nanotubes from a simple protein building block

In vitro self-assembly of tailorable nanotubes from a simple protein building block

10.1073/pnas.0712247105

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We demonstrate a method for generating discretely structured protein nanotubes from the simple ring-shaped building block, homohexameric Hcp1 from Pseudomonas aeruginosa . Our design exploited the observation that the crystal lattice of Hcp1 contains rings stacked in a repeating head-to-tail pattern. High-resolution detail of the ring–ring interface allowed the selection of sites for specific cysteine mutations capable of engaging in disulfide bond formation across rings, thereby generating stable Hcp1 nanotubes. Protein nanotubes containing up to 25 subunits (≈100 nm in length) were self-assembled under simple conditions. Furthermore, we demonstrate that the tube ends and interior can be independently and specifically functionalized to generate nanocapsules.

Bibliography

Ballister, E. R., Lai, A. H., Zuckermann, R. N., Cheng, Y., & Mougous, J. D. (2008). In vitro self-assembly of tailorable nanotubes from a simple protein building block. Proceedings of the National Academy of Sciences, 105(10), 3733â3738.

Authors 5

Edward R. Ballister (first)
Angela H. Lai (additional)
Ronald N. Zuckermann (additional)
Yifan Cheng (additional)
Joseph D. Mougous (additional)

References 30 Referenced 264

Dates

Type	When
Created	17 years, 5 months ago (Feb. 29, 2008, 9:36 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 5:02 p.m.)
Indexed	3 weeks, 4 days ago (Aug. 2, 2025, 12:41 a.m.)
Issued	17 years, 5 months ago (March 11, 2008)
Published	17 years, 5 months ago (March 11, 2008)
Published Online	17 years, 5 months ago (March 11, 2008)
Published Print	17 years, 5 months ago (March 11, 2008)

Funders 0

None

BibTeX

@article{Ballister_2008, title={In vitro self-assembly of tailorable nanotubes from a simple protein building block}, volume={105}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0712247105}, DOI={10.1073/pnas.0712247105}, number={10}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Ballister, Edward R. and Lai, Angela H. and Zuckermann, Ronald N. and Cheng, Yifan and Mougous, Joseph D.}, year={2008}, month=mar, pages={3733–3738} }

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