DOI: 10.1073/pnas.0711183105. Transgenic expression of<i>Helicobacter pylori</i>CagA induces gastrointestinal and hematopoietic neoplasms in mouse

Transgenic expression ofHelicobacter pyloriCagA induces gastrointestinal and hematopoietic neoplasms in mouse

10.1073/pnas.0711183105

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Infection withcagA-positiveHelicobacter pyloriis associated with gastric adenocarcinoma and gastric mucosa-associated lymphoid tissue (MALT) lymphoma of B cell origin. ThecagA-encoded CagA protein is delivered into gastric epithelial cells via the bacterial type IV secretion system and, upon tyrosine phosphorylation by Src family kinases, specifically binds to and aberrantly activates SHP-2 tyrosine phosphatase, a bona fide oncoprotein in human malignancies. CagA also elicits junctional and polarity defects in epithelial cells by interacting with and inhibiting partitioning-defective 1 (PAR1)/microtubule affinity-regulating kinase (MARK) independently of CagA tyrosine phosphorylation. Despite these CagA activities that contribute to neoplastic transformation, a causal link between CagA andin vivooncogenesis remains unknown. Here, we generated transgenic mice expressing wild-type or phosphorylation-resistant CagA throughout the body or predominantly in the stomach. Wild-type CagA transgenic mice showed gastric epithelial hyperplasia and some of the mice developed gastric polyps and adenocarcinomas of the stomach and small intestine. Systemic expression of wild-type CagA further induced leukocytosis with IL-3/GM-CSF hypersensitivity and some mice developed myeloid leukemias and B cell lymphomas, the hematological malignancies also caused by gain-of-function SHP-2 mutations. Such pathological abnormalities were not observed in transgenic mice expressing phosphorylation-resistant CagA. These results provide first direct evidence for the role of CagA as a bacterium-derived oncoprotein (bacterial oncoprotein) that acts in mammals and further indicate the importance of CagA tyrosine phosphorylation, which enables CagA to deregulate SHP-2, in the development ofH. pylori-associated neoplasms.

Bibliography

Ohnishi, N., Yuasa, H., Tanaka, S., Sawa, H., Miura, M., Matsui, A., Higashi, H., Musashi, M., Iwabuchi, K., Suzuki, M., Yamada, G., Azuma, T., & Hatakeyama, M. (2008). Transgenic expression ofHelicobacter pyloriCagA induces gastrointestinal and hematopoietic neoplasms in mouse. Proceedings of the National Academy of Sciences, 105(3), 1003â1008.

Authors 13

Naomi Ohnishi (first)
Hitomi Yuasa (additional)
Shinya Tanaka (additional)
Hirofumi Sawa (additional)
Motohiro Miura (additional)
Atsushi Matsui (additional)
Hideaki Higashi (additional)
Manabu Musashi (additional)
Kazuya Iwabuchi (additional)
Misao Suzuki (additional)
Gen Yamada (additional)
Takeshi Azuma (additional)
Masanori Hatakeyama (additional)

References 40 Referenced 488

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Dates

Type	When
Created	17 years, 7 months ago (Jan. 11, 2008, 8:53 p.m.)
Deposited	7 months, 1 week ago (Jan. 25, 2025, 11:03 a.m.)
Indexed	1 week, 1 day ago (Aug. 28, 2025, 8:44 a.m.)
Issued	17 years, 7 months ago (Jan. 22, 2008)
Published	17 years, 7 months ago (Jan. 22, 2008)
Published Online	17 years, 7 months ago (Jan. 22, 2008)
Published Print	17 years, 7 months ago (Jan. 22, 2008)

Funders 0

None

BibTeX

@article{Ohnishi_2008, title={Transgenic expression ofHelicobacter pyloriCagA induces gastrointestinal and hematopoietic neoplasms in mouse}, volume={105}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0711183105}, DOI={10.1073/pnas.0711183105}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Ohnishi, Naomi and Yuasa, Hitomi and Tanaka, Shinya and Sawa, Hirofumi and Miura, Motohiro and Matsui, Atsushi and Higashi, Hideaki and Musashi, Manabu and Iwabuchi, Kazuya and Suzuki, Misao and Yamada, Gen and Azuma, Takeshi and Hatakeyama, Masanori}, year={2008}, month=jan, pages={1003–1008} }

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