DOI: 10.1073/pnas.0708066104. Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories

Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories

10.1073/pnas.0708066104

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Organization in biological membranes spans many orders of magnitude in length scale, but limited resolution in far-field light microscopy has impeded distinction between numerous biomembrane models. One canonical example of a heterogeneously distributed membrane protein is hemagglutinin (HA) from influenza virus, which is associated with controversial cholesterol-rich lipid rafts. Using fluorescence photoactivation localization microscopy, we are able to image distributions of tens of thousands of HA molecules with subdiffraction resolution (≈40 nm) in live and fixed fibroblasts. HA molecules form irregular clusters on length scales from ≈40 nm up to many micrometers, consistent with results from electron microscopy. In live cells, the dynamics of HA molecules within clusters is observed and quantified to determine an effective diffusion coefficient. The results are interpreted in terms of several established models of biological membranes.

Bibliography

Hess, S. T., Gould, T. J., Gudheti, M. V., Maas, S. A., Mills, K. D., & Zimmerberg, J. (2007). Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories. Proceedings of the National Academy of Sciences, 104(44), 17370â17375.

Authors 6

Samuel T. Hess (first)
Travis J. Gould (additional)
Manasa V. Gudheti (additional)
Sarah A. Maas (additional)
Kevin D. Mills (additional)
Joshua Zimmerberg (additional)

References 32 Referenced 340

Dates

Type	When
Created	17 years, 10 months ago (Oct. 24, 2007, 7:20 p.m.)
Deposited	7 months ago (Jan. 21, 2025, 4:41 p.m.)
Indexed	4 weeks ago (July 30, 2025, 9:55 a.m.)
Issued	17 years, 9 months ago (Oct. 30, 2007)
Published	17 years, 9 months ago (Oct. 30, 2007)
Published Online	17 years, 9 months ago (Oct. 30, 2007)
Published Print	17 years, 9 months ago (Oct. 30, 2007)

Funders 0

None

BibTeX

@article{Hess_2007, title={Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories}, volume={104}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0708066104}, DOI={10.1073/pnas.0708066104}, number={44}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hess, Samuel T. and Gould, Travis J. and Gudheti, Manasa V. and Maas, Sarah A. and Mills, Kevin D. and Zimmerberg, Joshua}, year={2007}, month=oct, pages={17370–17375} }

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