DOI: 10.1073/pnas.0702731104. p120 catenin regulates lamellipodial dynamics and cell adhesion in cooperation with cortactin

p120 catenin regulates lamellipodial dynamics and cell adhesion in cooperation with cortactin

10.1073/pnas.0702731104

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The armadillo-family protein, p120 catenin (p120), binds to the juxtamembrane domain of classical cadherins and increases cell–cell junction stability. Overexpression of p120 modulates the activity of Rho family GTPases and augments cell migratory ability. Here we show that down-regulation of p120 in epithelial MCF-7 cells by siRNA leads to a striking decrease in lamellipodial persistence and focal adhesion formation. Similar alterations in lamellipodial activity were observed in MCF-7 cells treated with siRNA to cortactin, an activator of Arp2/3-dependent actin polymerization. We found that, in many cell types, p120 is colocalized with cortactin-containing actin structures not only at cell–cell junctions, but also at extrajunctional sites including membrane ruffles and actin-rich halos around endocytotic vesicles. p120 depletion led to dramatic loss of cortactin and its partner, Arp3, from the cell leading edges. Cortactin and p120 are shown to directly interact with each other via the cortactin N-terminal region. We propose that the mechanism underlying p120 functions at the leading edge involves its cooperation with cortactin.

Bibliography

Boguslavsky, S., Grosheva, I., Landau, E., Shtutman, M., Cohen, M., Arnold, K., Feinstein, E., Geiger, B., & Bershadsky, A. (2007). p120 catenin regulates lamellipodial dynamics and cell adhesion in cooperation with cortactin. Proceedings of the National Academy of Sciences, 104(26), 10882â10887.

Authors 9

Shlomit Boguslavsky (first)
Inna Grosheva (additional)
Elad Landau (additional)
Michael Shtutman (additional)
Miriam Cohen (additional)
Katya Arnold (additional)
Elena Feinstein (additional)
Benjamin Geiger (additional)
Alexander Bershadsky (additional)

References 48 Referenced 77

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Dates

Type	When
Created	18 years, 2 months ago (June 18, 2007, 9:11 p.m.)
Deposited	1 year, 6 months ago (Feb. 14, 2024, 10:55 p.m.)
Indexed	4 months, 2 weeks ago (April 19, 2025, 12:10 a.m.)
Issued	18 years, 2 months ago (June 26, 2007)
Published	18 years, 2 months ago (June 26, 2007)
Published Online	18 years, 2 months ago (June 26, 2007)
Published Print	18 years, 2 months ago (June 26, 2007)

Funders 0

None

BibTeX

@article{Boguslavsky_2007, title={p120 catenin regulates lamellipodial dynamics and cell adhesion in cooperation with cortactin}, volume={104}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0702731104}, DOI={10.1073/pnas.0702731104}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Boguslavsky, Shlomit and Grosheva, Inna and Landau, Elad and Shtutman, Michael and Cohen, Miriam and Arnold, Katya and Feinstein, Elena and Geiger, Benjamin and Bershadsky, Alexander}, year={2007}, month=jun, pages={10882–10887} }

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