DOI: 10.1073/pnas.0701291104. Genome sequencing and comparative analysis of <i>Saccharomyces cerevisiae</i> strain YJM789

Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789

10.1073/pnas.0701291104

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We sequenced the genome of Saccharomyces cerevisiae strain YJM789, which was derived from a yeast isolated from the lung of an AIDS patient with pneumonia. The strain is used for studies of fungal infections and quantitative genetics because of its extensive phenotypic differences to the laboratory reference strain, including growth at high temperature and deadly virulence in mouse models. Here we show that the ≈12-Mb genome of YJM789 contains ≈60,000 SNPs and ≈6,000 indels with respect to the reference S288c genome, leading to protein polymorphisms with a few known cases of phenotypic changes. Several ORFs are found to be unique to YJM789, some of which might have been acquired through horizontal transfer. Localized regions of high polymorphism density are scattered over the genome, in some cases spanning multiple ORFs and in others concentrated within single genes. The sequence of YJM789 contains clues to pathogenicity and spurs the development of more powerful approaches to dissecting the genetic basis of complex hereditary traits.

Bibliography

Wei, W., McCusker, J. H., Hyman, R. W., Jones, T., Ning, Y., Cao, Z., Gu, Z., Bruno, D., Miranda, M., Nguyen, M., Wilhelmy, J., Komp, C., Tamse, R., Wang, X., Jia, P., Luedi, P., Oefner, P. J., David, L., Dietrich, F. S., â¦ Steinmetz, L. M. (2007). Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789. Proceedings of the National Academy of Sciences, 104(31), 12825â12830.

Authors 22

Wu Wei (first)
John H. McCusker (additional)
Richard W. Hyman (additional)
Ted Jones (additional)
Ye Ning (additional)
Zhiwei Cao (additional)
Zhenglong Gu (additional)
Dan Bruno (additional)
Molly Miranda (additional)
Michelle Nguyen (additional)
Julie Wilhelmy (additional)
Caridad Komp (additional)
Raquel Tamse (additional)
Xiaojing Wang (additional)
Peilin Jia (additional)
Philippe Luedi (additional)
Peter J. Oefner (additional)
Lior David (additional)
Fred S. Dietrich (additional)
Yixue Li (additional)
Ronald W. Davis (additional)
Lars M. Steinmetz (additional)

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Dates

Type	When
Created	18 years, 1 month ago (July 25, 2007, 9:19 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 4:28 p.m.)
Indexed	1 month, 1 week ago (July 14, 2025, 11:20 p.m.)
Issued	18 years ago (July 31, 2007)
Published	18 years ago (July 31, 2007)
Published Online	18 years ago (July 31, 2007)
Published Print	18 years ago (July 31, 2007)

Funders 0

None

BibTeX

@article{Wei_2007, title={Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789}, volume={104}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0701291104}, DOI={10.1073/pnas.0701291104}, number={31}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wei, Wu and McCusker, John H. and Hyman, Richard W. and Jones, Ted and Ning, Ye and Cao, Zhiwei and Gu, Zhenglong and Bruno, Dan and Miranda, Molly and Nguyen, Michelle and Wilhelmy, Julie and Komp, Caridad and Tamse, Raquel and Wang, Xiaojing and Jia, Peilin and Luedi, Philippe and Oefner, Peter J. and David, Lior and Dietrich, Fred S. and Li, Yixue and Davis, Ronald W. and Steinmetz, Lars M.}, year={2007}, month=jul, pages={12825–12830} }

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