DOI: 10.1073/pnas.0700629104. Structural basis for reversible photoswitching in Dronpa

Structural basis for reversible photoswitching in Dronpa

10.1073/pnas.0700629104

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Dronpa is a novel GFP-like fluorescent protein with exceptional light-controlled switching properties. It may be reversibly switched between a fluorescent on-state and a nonfluorescent off-state by irradiation with light. To elucidate the molecular basis of the switching mechanism, we generated reversibly switchable Dronpa protein crystals. Using these crystals we determined the elusive dark-state structure of Dronpa at 1.95-Å resolution. We found that the photoswitching results in a cis–trans isomerization of the chromophore accompanied by complex structural rearrangements of four nearby amino acid residues. Because of this cascade of intramolecular events, the chromophore is exposed to distinct electrostatic surface potentials, which are likely to influence the protonation equilibria at the chromophore. We suggest a comprehensive model for the light-induced switching mechanism, connecting a cascade of structural rearrangements with different protonation states of the chromophore.

Bibliography

Andresen, M., Stiel, A. C., Trowitzsch, S., Weber, G., Eggeling, C., Wahl, M. C., Hell, S. W., & Jakobs, S. (2007). Structural basis for reversible photoswitching in Dronpa. Proceedings of the National Academy of Sciences, 104(32), 13005â13009.

Authors 8

Martin Andresen (first)
Andre C. Stiel (additional)
Simon Trowitzsch (additional)
Gert Weber (additional)
Christian Eggeling (additional)
Markus C. Wahl (additional)
Stefan W. Hell (additional)
Stefan Jakobs (additional)

References 31 Referenced 252

Dates

Type	When
Created	18 years, 1 month ago (July 23, 2007, 9:18 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 3:08 p.m.)
Indexed	48 minutes ago (Aug. 27, 2025, 6:27 a.m.)
Issued	18 years ago (Aug. 7, 2007)
Published	18 years ago (Aug. 7, 2007)
Published Online	18 years ago (Aug. 7, 2007)
Published Print	18 years ago (Aug. 7, 2007)

Funders 0

None

BibTeX

@article{Andresen_2007, title={Structural basis for reversible photoswitching in Dronpa}, volume={104}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0700629104}, DOI={10.1073/pnas.0700629104}, number={32}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Andresen, Martin and Stiel, Andre C. and Trowitzsch, Simon and Weber, Gert and Eggeling, Christian and Wahl, Markus C. and Hell, Stefan W. and Jakobs, Stefan}, year={2007}, month=aug, pages={13005–13009} }

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