DOI: 10.1073/pnas.0634629100. Protein pathway and complex clustering of correlated mRNA and protein expression analyses in <i>Saccharomyces</i> <i>cerevisiae</i>

Protein pathway and complex clustering of correlated mRNA and protein expression analyses in Saccharomyces cerevisiae

10.1073/pnas.0634629100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The mRNA and protein expression in Saccharomyces cerevisiae cultured in rich or minimal media was analyzed by oligonucleotide arrays and quantitative multidimensional protein identification technology. The overall correlation between mRNA and protein expression was weakly positive with a Spearman rank correlation coefficient of 0.45 for 678 loci. To place the data sets in a proper biological context, a clustering approach based on protein pathways and protein complexes was implemented. Protein expression levels were transcriptionally controlled for not only single loci but for entire protein pathways (e.g., Met, Arg, and Leu biosynthetic pathways). In contrast, the protein expression of loci in several protein complexes (e.g., SPT, COPI, and ribosome) was posttranscriptionally controlled. The coupling of the methods described provided insight into the biology of S. cerevisiae and a clustering strategy by which future studies should be based.

Bibliography

Washburn, M. P., Koller, A., Oshiro, G., Ulaszek, R. R., Plouffe, D., Deciu, C., Winzeler, E., & Yates, J. R. (2003). Protein pathway and complex clustering of correlated mRNA and protein expression analyses in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences, 100(6), 3107â3112.

Authors 8

Michael P. Washburn (first)
Antonius Koller (additional)
Guy Oshiro (additional)
Ryan R. Ulaszek (additional)
David Plouffe (additional)
Cosmin Deciu (additional)
Elizabeth Winzeler (additional)
John R. Yates (additional)

References 44 Referenced 304

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A G Hinnebusch The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, eds E W Jones, J R Pringle, J R Broach (Cold Spring Harbor Lab. Press, Plainview, NY) 2, 319–414 (1992). / The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression by Hinnebusch A G (1992)
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Dates

Type	When
Created	22 years, 5 months ago (March 18, 2003, 6:15 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 3:27 p.m.)
Indexed	4 days, 2 hours ago (Aug. 26, 2025, 3:09 a.m.)
Issued	22 years, 5 months ago (March 7, 2003)
Published	22 years, 5 months ago (March 7, 2003)
Published Online	22 years, 5 months ago (March 7, 2003)
Published Print	22 years, 5 months ago (March 18, 2003)

Funders 0

None

BibTeX

@article{Washburn_2003, title={Protein pathway and complex clustering of correlated mRNA and protein expression analyses in Saccharomyces cerevisiae}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0634629100}, DOI={10.1073/pnas.0634629100}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Washburn, Michael P. and Koller, Antonius and Oshiro, Guy and Ulaszek, Ryan R. and Plouffe, David and Deciu, Cosmin and Winzeler, Elizabeth and Yates, John R.}, year={2003}, month=mar, pages={3107–3112} }

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