DOI: 10.1073/pnas.0610297104. Structural analysis and dimerization potential of the human TAF5 subunit of TFIID

Structural analysis and dimerization potential of the human TAF5 subunit of TFIID

10.1073/pnas.0610297104

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

TFIID is an essential factor required for RNA polymerase II transcription but remains poorly understood because of its intrinsic complexity. Human TAF5, a 100-kDa subunit of general transcription factor TFIID, is an essential gene and plays a critical role in assembling the 1.2 MDa TFIID complex. We report here a structural analysis of the TAF5 protein. Our structure at 2.2-Å resolution of the TAF5-NTD2 domain reveals an α-helical domain with distant structural similarity to RNA polymerase II CTD interacting factors. The TAF5-NTD2 domain contains several conserved clefts likely to be critical for TFIID complex assembly. Our biochemical analysis of the human TAF5 protein demonstrates the ability of the N-terminal half of the TAF5 gene to form a flexible, extended dimer, a key property required for the assembly of the TFIID complex.

Bibliography

Bhattacharya, S., Takada, S., & Jacobson, R. H. (2007). Structural analysis and dimerization potential of the human TAF5 subunit of TFIID. Proceedings of the National Academy of Sciences, 104(4), 1189â1194.

Authors 3

Suparna Bhattacharya (first)
Shinako Takada (additional)
Raymond H. Jacobson (additional)

References 41 Referenced 25

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Dates

Type	When
Created	18 years, 7 months ago (Jan. 16, 2007, 10:18 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 2:45 p.m.)
Indexed	2 months ago (June 28, 2025, 10:22 a.m.)
Issued	18 years, 7 months ago (Jan. 23, 2007)
Published	18 years, 7 months ago (Jan. 23, 2007)
Published Online	18 years, 7 months ago (Jan. 23, 2007)
Published Print	18 years, 7 months ago (Jan. 23, 2007)

Funders 0

None

BibTeX

@article{Bhattacharya_2007, title={Structural analysis and dimerization potential of the human TAF5 subunit of TFIID}, volume={104}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0610297104}, DOI={10.1073/pnas.0610297104}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Bhattacharya, Suparna and Takada, Shinako and Jacobson, Raymond H.}, year={2007}, month=jan, pages={1189–1194} }

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