DOI: 10.1073/pnas.0609643104. Wide-field subdiffraction imaging by accumulated binding of diffusing probes [Penn]

Wide-field subdiffraction imaging by accumulated binding of diffusing probes

10.1073/pnas.0609643104

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A method is introduced for subdiffraction imaging that accumulates points by collisional flux. It is based on targeting the surface of objects by fluorescent probes diffusing in the solution. Because the flux of probes at the object is essentially constant over long time periods, the examination of an almost unlimited number of individual probe molecules becomes possible. Each probe that hits the object and that becomes immobilized is located with high precision by replacing its point-spread function by a point at its centroid. Images of lipid bilayers, contours of these bilayers, and large unilamellar vesicles are shown. A spatial resolution of ≈25 nm is readily achieved. The ability of the method to effect rapid nanoscale imaging and spatial resolution below Rayleigh criterion and without the necessity for labeling with fluorescent probes is proven.

Bibliography

Sharonov, A., & Hochstrasser, R. M. (2006). Wide-field subdiffraction imaging by accumulated binding of diffusing probes. Proceedings of the National Academy of Sciences, 103(50), 18911â18916.

Authors 2

Alexey Sharonov (first)
Robin M. Hochstrasser (additional)

References 24 Referenced 1,028

10.1002/anie.200300647
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Dates

Type	When
Created	18 years, 8 months ago (Dec. 1, 2006, 8:34 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 3:01 p.m.)
Indexed	1 day, 20 hours ago (Aug. 30, 2025, 12:55 p.m.)
Issued	18 years, 8 months ago (Dec. 12, 2006)
Published	18 years, 8 months ago (Dec. 12, 2006)
Published Online	18 years, 8 months ago (Dec. 12, 2006)
Published Print	18 years, 8 months ago (Dec. 12, 2006)

Funders 0

None

BibTeX

@article{Sharonov_2006, title={Wide-field subdiffraction imaging by accumulated binding of diffusing probes}, volume={103}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0609643104}, DOI={10.1073/pnas.0609643104}, number={50}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sharonov, Alexey and Hochstrasser, Robin M.}, year={2006}, month=dec, pages={18911–18916} }

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