DOI: 10.1073/pnas.0609009103. Plasma membrane-associated proteins are clustered into islands attached to the cytoskeleton

Plasma membrane-associated proteins are clustered into islands attached to the cytoskeleton

10.1073/pnas.0609009103

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Although much evidence suggests that the plasma membrane of eukaryotic cells is not homogenous, the precise architecture of this important structure has not been clear. Here we use transmission electron microscopy of plasma membrane sheets and specific probes to show that most or all plasma membrane-associated proteins are clustered in cholesterol-enriched domains (“islands”) that are separated by “protein-free” and cholesterol-low membrane. These islands are further divided into subregions, as shown by the localization of “raft” and “non-raft” markers to specific areas. Abundant actin staining and inhibitor studies show that these structures are connected to the cytoskeleton and at least partially depend on it for their formation and/or maintenance.

Bibliography

Lillemeier, B. F., Pfeiffer, J. R., Surviladze, Z., Wilson, B. S., & Davis, M. M. (2006). Plasma membrane-associated proteins are clustered into islands attached to the cytoskeleton. Proceedings of the National Academy of Sciences, 103(50), 18992â18997.

Authors 5

Björn F. Lillemeier (first)
Janet R. Pfeiffer (additional)
Zurab Surviladze (additional)
Bridget S. Wilson (additional)
Mark M. Davis (additional)

References 29 Referenced 340

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Dates

Type	When
Created	18 years, 8 months ago (Dec. 4, 2006, 8:39 p.m.)
Deposited	7 months, 1 week ago (Jan. 12, 2025, 1:51 a.m.)
Indexed	5 days, 4 hours ago (Aug. 20, 2025, 8:22 a.m.)
Issued	18 years, 8 months ago (Dec. 12, 2006)
Published	18 years, 8 months ago (Dec. 12, 2006)
Published Online	18 years, 8 months ago (Dec. 12, 2006)
Published Print	18 years, 8 months ago (Dec. 12, 2006)

Funders 0

None

BibTeX

@article{Lillemeier_2006, title={Plasma membrane-associated proteins are clustered into islands attached to the cytoskeleton}, volume={103}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0609009103}, DOI={10.1073/pnas.0609009103}, number={50}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Lillemeier, Björn F. and Pfeiffer, Janet R. and Surviladze, Zurab and Wilson, Bridget S. and Davis, Mark M.}, year={2006}, month=dec, pages={18992–18997} }

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