DOI: 10.1073/pnas.0607541103. Structural basis for mRNA and tRNA positioning on the ribosome

Structural basis for mRNA and tRNA positioning on the ribosome

10.1073/pnas.0607541103

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Protein synthesis requires the accurate positioning of mRNA and tRNA in the peptidyl-tRNA site of the ribosome. Here we describe x-ray crystal structures of the intact bacterial ribosome from Escherichia coli in a complex with mRNA and the anticodon stem-loop of P-site tRNA. At 3.5-Å resolution, these structures reveal rearrangements in the intact ribosome that clamp P-site tRNA and mRNA on the small ribosomal subunit. Binding of the anticodon stem-loop of P-site tRNA to the ribosome is sufficient to lock the head of the small ribosomal subunit in a single conformation, thereby preventing movement of mRNA and tRNA before mRNA decoding.

Bibliography

Berk, V., Zhang, W., Pai, R. D., & Cate, J. H. D. (2006). Structural basis for mRNA and tRNA positioning on the ribosome. Proceedings of the National Academy of Sciences, 103(43), 15830â15834.

Authors 4

Veysel Berk (first)
Wen Zhang (additional)
Raj D. Pai (additional)
Jamie H. D. Cate (additional)

References 32 Referenced 150

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 13, 2006, 6:36 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 2:38 p.m.)
Indexed	3 weeks ago (Aug. 2, 2025, 1:14 a.m.)
Issued	18 years, 9 months ago (Oct. 24, 2006)
Published	18 years, 9 months ago (Oct. 24, 2006)
Published Online	18 years, 9 months ago (Oct. 24, 2006)
Published Print	18 years, 9 months ago (Oct. 24, 2006)

Funders 0

None

BibTeX

@article{Berk_2006, title={Structural basis for mRNA and tRNA positioning on the ribosome}, volume={103}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0607541103}, DOI={10.1073/pnas.0607541103}, number={43}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Berk, Veysel and Zhang, Wen and Pai, Raj D. and Cate, Jamie H. D.}, year={2006}, month=oct, pages={15830–15834} }

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