DOI: 10.1073/pnas.0606880104. Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex

Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex

10.1073/pnas.0606880104

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In the process of protein synthesis, the small (40S) subunit of the eukaryotic ribosome is recruited to the capped 5′ end of the mRNA, from which point it scans along the 5′ untranslated region in search of a start codon. However, the 40S subunit alone is not capable of functional association with cellular mRNA species; it has to be prepared for the recruitment and scanning steps by interactions with a group of eukaryotic initiation factors (eIFs). In budding yeast, an important subset of these factors ( 1 , 2 , 3 , and 5 ) can form a multifactor complex (MFC). Here, we describe cryo-EM reconstructions of the 40S subunit, of the MFC, and of 40S complexes with MFC factors plus eIF1A. These studies reveal the positioning of the core MFC on the 40S subunit, and show how eIF-binding induces mobility in the head and platform and reconfigures the head–platform–body relationship. This is expected to increase the accessibility of the mRNA channel, thus enabling the 40S subunit to convert to a recruitment-competent state.

Bibliography

Gilbert, R. J. C., Gordiyenko, Y., von der Haar, T., Sonnen, A. F.-P., Hofmann, G., Nardelli, M., Stuart, D. I., & McCarthy, J. E. G. (2007). Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex. Proceedings of the National Academy of Sciences, 104(14), 5788â5793.

Authors 8

Robert J. C. Gilbert (first)
Yulya Gordiyenko (additional)
Tobias von der Haar (additional)
Andreas F.-P. Sonnen (additional)
Gregor Hofmann (additional)
Maria Nardelli (additional)
David I. Stuart (additional)
John E. G. McCarthy (additional)

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Dates

Type	When
Created	18 years, 5 months ago (March 27, 2007, 11:41 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 3:35 p.m.)
Indexed	3 months, 4 weeks ago (May 1, 2025, 11:41 a.m.)
Issued	18 years, 4 months ago (April 3, 2007)
Published	18 years, 4 months ago (April 3, 2007)
Published Online	18 years, 4 months ago (April 3, 2007)
Published Print	18 years, 4 months ago (April 3, 2007)

Funders 0

None

BibTeX

@article{Gilbert_2007, title={Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex}, volume={104}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0606880104}, DOI={10.1073/pnas.0606880104}, number={14}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Gilbert, Robert J. C. and Gordiyenko, Yulya and von der Haar, Tobias and Sonnen, Andreas F.-P. and Hofmann, Gregor and Nardelli, Maria and Stuart, David I. and McCarthy, John E. G.}, year={2007}, month=apr, pages={5788–5793} }

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