DOI: 10.1073/pnas.0603337103. The intricate side of systems biology

10.1073/pnas.0603337103

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The combination of high-throughput methods of molecular biology with advanced mathematical and computational techniques has propelled the emergent field of systems biology into a position of prominence. Unthinkable a decade ago, it has become possible to screen and analyze the expression of entire genomes, simultaneously assess large numbers of proteins and their prevalence, and characterize in detail the metabolic state of a cell population. Although very important, the focus on comprehensive networks of biological components is only one side of systems biology. Complementing large-scale assessments, and sometimes at the risk of being forgotten, are more subtle analyses that rationalize the design and functioning of biological modules in exquisite detail. This intricate side of systems biology aims at identifying the specific roles of processes and signals in smaller, fully regulated systems by computing what would happen if these signals were lacking or organized in a different fashion. We exemplify this type of approach with a detailed analysis of the regulation of glucose utilization in Lactococcus lactis . This organism is exposed to alternating periods of glucose availability and starvation. During starvation, it accumulates an intermediate of glycolysis, which allows it to take up glucose immediately upon availability. This notable accumulation poses a nontrivial control task that is solved with an unusual, yet ingeniously designed and timed feedforward activation system. The elucidation of this control system required high-precision, dynamic in vivo metabolite data, combined with methods of nonlinear systems analysis, and may serve as a paradigm for multidisciplinary approaches to fine-scaled systems biology.

Bibliography

Voit, E., Neves, A. R., & Santos, H. (2006). The intricate side of systems biology. Proceedings of the National Academy of Sciences, 103(25), 9452â9457.

Authors 3

Eberhard Voit (first)
Ana Rute Neves (additional)
Helena Santos (additional)

References 20 Referenced 69

10.1002/(SICI)1097-0290(19990720)64:2<200::AID-BIT9>3.0.CO;2-K
10.1016/S0021-9258(19)69886-8
E. O. Voit Computational Analysis of Biochemical Systems. A Practical Guide for Biochemists and Molecular Biologists (Cambridge Univ. Press, Cambridge, U.K., 2000). / Computational Analysis of Biochemical Systems. A Practical Guide for Biochemists and Molecular Biologists by Voit E. O. (2000)
10.1016/j.fmrre.2005.04.005
10.1128/AEM.68.12.6332-6342.2002
M. A. Savageau Biochemical Systems Analysis. A Study of Function and Design in Molecular Biology (Addison–Wesley, Reading, MA, 1976). / Biochemical Systems Analysis. A Study of Function and Design in Molecular Biology by Savageau M. A. (1976)
10.1016/0022-5193(67)90045-8
10.1016/B978-0-12-152801-0.50007-9
10.1111/j.1574-6968.1987.tb02462.x
10.1099/mic.0.26695-0
10.1016/0005-2744(76)90225-4
10.1016/S0764-4469(00)01295-6
10.1016/0022-5193(79)90016-X
10.1016/0022-5193(79)90017-1
10.1016/S0022-5193(69)80026-3
10.1017/CBO9780511546334
10.1038/nature03232
E. O. Voit, J. S. Almeida, S. Marino, R. Lall, G. Goel, A. R. Neves, H. Santos IEE Proc. Syst. Biol, in press. (2006). / IEE Proc. Syst. Biol by Voit E. O. (2006)
S. Marino, E. O. Voit J. Bioinform. Comp. Biol, in press. (2006). / J. Bioinform. Comp. Biol by Marino S. (2006)
10.1016/0141-0229(90)90033-M

Dates

Type	When
Created	19 years, 2 months ago (June 9, 2006, 8:48 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 3:50 p.m.)
Indexed	1 year, 2 months ago (May 31, 2024, 3:13 p.m.)
Issued	19 years, 2 months ago (June 20, 2006)
Published	19 years, 2 months ago (June 20, 2006)
Published Online	19 years, 2 months ago (June 20, 2006)
Published Print	19 years, 2 months ago (June 20, 2006)

Funders 0

None

BibTeX

@article{Voit_2006, title={The intricate side of systems biology}, volume={103}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0603337103}, DOI={10.1073/pnas.0603337103}, number={25}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Voit, Eberhard and Neves, Ana Rute and Santos, Helena}, year={2006}, month=jun, pages={9452–9457} }

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