DOI: 10.1073/pnas.0602766103. Time scales of membrane fusion revealed by direct imaging of vesicle fusion with high temporal resolution

Time scales of membrane fusion revealed by direct imaging of vesicle fusion with high temporal resolution

10.1073/pnas.0602766103

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Membrane fusion is a vital process of life involved, for example, in cellular secretion via exocytosis, signaling between nerve cells, and virus infection. In both the life sciences and bioengineering, controlled membrane fusion has many possible applications, such as drug delivery, gene transfer, chemical microreactors, or synthesis of nanomaterials. Until now, the fusion dynamics has been elusive because direct observations have been limited to time scales that exceed several milliseconds. Here, the fusion of giant lipid vesicles is induced in a controlled manner and monitored with a temporal resolution of 50 μs. Two different fusion protocols are used that are based on synthetic fusogenic molecules and electroporation. For both protocols, the opening of the fusion necks is very fast, with an average expansion velocity of centimeters per second. This velocity indicates that the initial formation of a single fusion neck can be completed in a few hundred nanoseconds.

Bibliography

Haluska, C. K., Riske, K. A., Marchi-Artzner, V., Lehn, J.-M., Lipowsky, R., & Dimova, R. (2006). Time scales of membrane fusion revealed by direct imaging of vesicle fusion with high temporal resolution. Proceedings of the National Academy of Sciences, 103(43), 15841â15846.

Authors 6

Christopher K. Haluska (first)
Karin A. Riske (additional)
Valérie Marchi-Artzner (additional)
Jean-Marie Lehn (additional)
Reinhard Lipowsky (additional)
Rumiana Dimova (additional)

References 35 Referenced 210

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 17, 2006, 8:10 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 2:40 p.m.)
Indexed	1 day, 13 hours ago (Aug. 31, 2025, 6:04 a.m.)
Issued	18 years, 10 months ago (Oct. 24, 2006)
Published	18 years, 10 months ago (Oct. 24, 2006)
Published Online	18 years, 10 months ago (Oct. 24, 2006)
Published Print	18 years, 10 months ago (Oct. 24, 2006)

Funders 0

None

BibTeX

@article{Haluska_2006, title={Time scales of membrane fusion revealed by direct imaging of vesicle fusion with high temporal resolution}, volume={103}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0602766103}, DOI={10.1073/pnas.0602766103}, number={43}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Haluska, Christopher K. and Riske, Karin A. and Marchi-Artzner, Valérie and Lehn, Jean-Marie and Lipowsky, Reinhard and Dimova, Rumiana}, year={2006}, month=oct, pages={15841–15846} }

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