DOI: 10.1073/pnas.0536251100. Domain organization of the type 1 inositol 1,4,5-trisphosphate receptor as revealed by single-particle analysis

Domain organization of the type 1 inositol 1,4,5-trisphosphate receptor as revealed by single-particle analysis

10.1073/pnas.0536251100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The inositol 1,4,5-trisphosphate receptor (IP 3 R) is a tetrameric intracellular Ca 2+ channel, which mediates the release of Ca 2+ from the endoplasmic reticulum in response to many different extracellular stimuli. We present a 3D structure of the type 1 IP 3 R obtained by electron microscopy and single-particle analysis that reveals its domain organization. The IP 3 R has a flower-like appearance with fourfold symmetry and is made up of three distinct domains connected by slender links. By relating the organization of the structural domains to secondary-structure predictions and biochemical data we develop a model in which structural domains are mapped onto the amino acid sequence to deduce the location of the channel region and the cytoplasmic inositol 1,4,5-trisphosphate-binding and modulatory subdomains. The structure of the IP 3 R is compared with that of other tetrameric cation channels. The channel domain is similar in size and shape to its counterparts in the ryanodine receptor and the Shaker voltage-gated K + channel.

Bibliography

da Fonseca, P. C. A., Morris, S. A., Nerou, E. P., Taylor, C. W., & Morris, E. P. (2003). Domain organization of the type 1 inositol 1,4,5-trisphosphate receptor as revealed by single-particle analysis. Proceedings of the National Academy of Sciences, 100(7), 3936â3941.

Authors 5

Paula C. A. da Fonseca (first)
Stephen A. Morris (additional)
Edmund P. Nerou (additional)
Colin W. Taylor (additional)
Edward P. Morris (additional)

References 42 Referenced 77

Dates

Type	When
Created	22 years, 4 months ago (April 1, 2003, 2:25 p.m.)
Deposited	3 years, 4 months ago (April 25, 2022, 10:05 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 6, 2024, 7:52 p.m.)
Issued	22 years, 5 months ago (March 21, 2003)
Published	22 years, 5 months ago (March 21, 2003)
Published Online	22 years, 5 months ago (March 21, 2003)
Published Print	22 years, 4 months ago (April 1, 2003)

Funders 0

None

BibTeX

@article{da_Fonseca_2003, title={Domain organization of the type 1 inositol 1,4,5-trisphosphate receptor as revealed by single-particle analysis}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0536251100}, DOI={10.1073/pnas.0536251100}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={da Fonseca, Paula C. A. and Morris, Stephen A. and Nerou, Edmund P. and Taylor, Colin W. and Morris, Edward P.}, year={2003}, month=mar, pages={3936–3941} }

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