DOI: 10.1073/pnas.052018099. The role of the dynamics of focal adhesion kinase in the mechanotaxis of endothelial cells

The role of the dynamics of focal adhesion kinase in the mechanotaxis of endothelial cells

10.1073/pnas.052018099

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The migration of vascular endothelial cells (ECs) is critical in vascular remodeling. We showed that fluid shear stress enhanced EC migration in flow direction and called this “mechanotaxis.” To visualize the molecular dynamics of focal adhesion kinase (FAK) at focal adhesions (FAs), FAK tagged with green fluorescence protein (GFP) was expressed in ECs. Within 10 min of shear stress application, lamellipodial protrusion was induced at cell periphery in the flow direction, with the recruitment of FAK at FAs. ECs under flow migrated with polarized formation of new FAs in flow direction, and these newly formed FAs subsequently disassembled after the rear of the cell moved over them. The cells migrating under flow had a decreased number of FAs. In contrast to shear stress, serum did not significantly affect the speed of cell migration. Serum induced lamellipodia and FAK recruitment at FAs without directional preference. FAK(Y397) phosphorylation colocalized with GFP-FAK at FAs in both shear stress and serum experiments. The total level of FAK(Y397) phosphorylation after shear stress was lower than that after serum treatment, suggesting that the polarized change at cell periphery rather than the total level of FAK(Y397) phosphorylation is important for directional migration. Our results demonstrate the dynamics of FAK at FAs during the directional migration of EC in response to mechanical force, and suggest that mechanotaxis is an important mechanism controlling EC migration.

Bibliography

Li, S., Butler, P., Wang, Y., Hu, Y., Han, D. C., Usami, S., Guan, J.-L., & Chien, S. (2002). The role of the dynamics of focal adhesion kinase in the mechanotaxis of endothelial cells. Proceedings of the National Academy of Sciences, 99(6), 3546â3551.

Authors 8

Song Li (first)
Peter Butler (additional)
Yingxiao Wang (additional)
Yingli Hu (additional)
Dong Cho Han (additional)
Shunichi Usami (additional)
Jun-Lin Guan (additional)
Shu Chien (additional)

References 47 Referenced 215

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:37 a.m.)
Deposited	3 years, 4 months ago (April 25, 2022, 9:08 p.m.)
Indexed	6 days, 22 hours ago (Aug. 23, 2025, 9:40 p.m.)
Issued	23 years, 5 months ago (March 12, 2002)
Published	23 years, 5 months ago (March 12, 2002)
Published Online	23 years, 5 months ago (March 12, 2002)
Published Print	23 years, 5 months ago (March 19, 2002)

Funders 0

None

BibTeX

@article{Li_2002, title={The role of the dynamics of focal adhesion kinase in the mechanotaxis of endothelial cells}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.052018099}, DOI={10.1073/pnas.052018099}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Li, Song and Butler, Peter and Wang, Yingxiao and Hu, Yingli and Han, Dong Cho and Usami, Shunichi and Guan, Jun-Lin and Chien, Shu}, year={2002}, month=mar, pages={3546–3551} }

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