DOI: 10.1073/pnas.051634598. Complete genomic sequence of <i>Pasteurella multocida</i> ,Pm70

Complete genomic sequence of Pasteurella multocida ,Pm70

10.1073/pnas.051634598

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We present here the complete genome sequence of a common avian clone of Pasteurella multocida , Pm70. The genome of Pm70 is a single circular chromosome 2,257,487 base pairs in length and contains 2,014 predicted coding regions, 6 ribosomal RNA operons, and 57 tRNAs. Genome-scale evolutionary analyses based on pairwise comparisons of 1,197 orthologous sequences between P. multocida , Haemophilus influenzae , and Escherichia coli suggest that P. multocida and H. influenzae diverged ≈270 million years ago and the γ subdivision of the proteobacteria radiated about 680 million years ago. Two previously undescribed open reading frames, accounting for ≈1% of the genome, encode large proteins with homology to the virulence-associated filamentous hemagglutinin of Bordetella pertussis . Consistent with the critical role of iron in the survival of many microbial pathogens, in silico and whole-genome microarray analyses identified more than 50 Pm70 genes with a potential role in iron acquisition and metabolism. Overall, the complete genomic sequence and preliminary functional analyses provide a foundation for future research into the mechanisms of pathogenesis and host specificity of this important multispecies pathogen.

Bibliography

May, B. J., Zhang, Q., Li, L. L., Paustian, M. L., Whittam, T. S., & Kapur, V. (2001). Complete genomic sequence of Pasteurella multocida ,Pm70. Proceedings of the National Academy of Sciences, 98(6), 3460â3465.

Authors 6

Barbara J. May (first)
Qing Zhang (additional)
Ling Ling Li (additional)
Michael L. Paustian (additional)
Thomas S. Whittam (additional)
Vivek Kapur (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:41 a.m.)
Deposited	3 years, 2 months ago (June 7, 2022, 1:34 a.m.)
Indexed	4 weeks ago (Aug. 6, 2025, 10:01 a.m.)
Issued	24 years, 5 months ago (March 6, 2001)
Published	24 years, 5 months ago (March 6, 2001)
Published Online	24 years, 5 months ago (March 6, 2001)
Published Print	24 years, 5 months ago (March 13, 2001)

Funders 0

None

BibTeX

@article{May_2001, title={Complete genomic sequence of Pasteurella multocida ,Pm70}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.051634598}, DOI={10.1073/pnas.051634598}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={May, Barbara J. and Zhang, Qing and Li, Ling Ling and Paustian, Michael L. and Whittam, Thomas S. and Kapur, Vivek}, year={2001}, month=mar, pages={3460–3465} }

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  "abstract": "<jats:p>\n            We present here the complete genome sequence of a common avian\n clone of\n            <jats:italic>Pasteurella multocida</jats:italic>\n            , Pm70. The genome of Pm70\n is a single circular chromosome 2,257,487 base pairs in length and\n contains 2,014 predicted coding regions, 6 ribosomal RNA operons, and\n 57 tRNAs. Genome-scale evolutionary analyses based on pairwise\n comparisons of 1,197 orthologous sequences between\n            <jats:italic>P.\n multocida</jats:italic>\n            ,\n            <jats:italic>Haemophilus influenzae</jats:italic>\n            , and\n            <jats:italic>Escherichia coli</jats:italic>\n            suggest that\n            <jats:italic>P.\n multocida</jats:italic>\n            and\n            <jats:italic>H. influenzae</jats:italic>\n            diverged \u2248270\n million years ago and the \u03b3 subdivision of the proteobacteria\n radiated about 680 million years ago. Two previously undescribed open\n reading frames, accounting for \u22481% of the genome, encode large\n proteins with homology to the virulence-associated filamentous\n hemagglutinin of\n            <jats:italic>Bordetella pertussis</jats:italic>\n            . Consistent with\n the critical role of iron in the survival of many microbial pathogens,\n            <jats:italic>in silico</jats:italic>\n            and whole-genome microarray analyses\n identified more than 50 Pm70 genes with a potential role in iron\n acquisition and metabolism. Overall, the complete genomic sequence and\n preliminary functional analyses provide a foundation for future\n research into the mechanisms of pathogenesis and host specificity of\n this important multispecies pathogen.\n          </jats:p>",
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