DOI: 10.1073/pnas.051631398. DC-SIGNR, a DC-SIGN homologue expressed in endothelial cells, binds to human and simian immunodeficiency viruses and activates infection in trans [Penn]

DC-SIGNR, a DC-SIGN homologue expressed in endothelial cells, binds to human and simian immunodeficiency viruses and activates infection in trans

10.1073/pnas.051631398

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

DC-SIGN, a C-type lectin expressed on the surface of dendritic cells (DCs), efficiently binds and transmits HIVs and simian immunodeficiency viruses to susceptible cells in trans. A DC-SIGN homologue, termed DC-SIGNR, has recently been described. Herein we show that DC-SIGNR, like DC-SIGN, can bind to multiple strains of HIV-1, HIV-2, and simian immunodeficiency virus and transmit these viruses to both T cell lines and human peripheral blood mononuclear cells. Binding of virus to DC-SIGNR was dependent on carbohydrate recognition. Immunostaining with a DC-SIGNR-specific antiserum showed that DC-SIGNR was expressed on sinusoidal endothelial cells in the liver and on endothelial cells in lymph node sinuses and placental villi. The presence of this efficient virus attachment factor on multiple endothelial cell types indicates that DC-SIGNR could play a role in the vertical transmission of primate lentiviruses, in the enabling of HIV to traverse the capillary endothelium in some organs, and in the presentation of virus to CD4-positive cells in multiple locations including lymph nodes.

Bibliography

PÃ¶hlmann, S., Soilleux, E. J., Baribaud, F., Leslie, G. J., Morris, L. S., Trowsdale, J., Lee, B., Coleman, N., & Doms, R. W. (2001). DC-SIGNR, a DC-SIGN homologue expressed in endothelial cells, binds to human and simian immunodeficiency viruses and activates infection in trans. Proceedings of the National Academy of Sciences, 98(5), 2670â2675.

Authors 9

Stefan Pöhlmann (first)
Elizabeth J. Soilleux (additional)
Frédéric Baribaud (additional)
George J. Leslie (additional)
Lesley S. Morris (additional)
John Trowsdale (additional)
Benhur Lee (additional)
Nicholas Coleman (additional)
Robert W. Doms (additional)

References 34 Referenced 277

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:36 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:19 a.m.)
Indexed	56 minutes ago (Sept. 3, 2025, 2:03 a.m.)
Issued	24 years, 6 months ago (Feb. 27, 2001)
Published	24 years, 6 months ago (Feb. 27, 2001)
Published Online	24 years, 6 months ago (Feb. 27, 2001)
Published Print	24 years, 6 months ago (Feb. 27, 2001)

Funders 0

None

BibTeX

@article{P_hlmann_2001, title={DC-SIGNR, a DC-SIGN homologue expressed in endothelial cells, binds to human and simian immunodeficiency viruses and activates infection in trans}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.051631398}, DOI={10.1073/pnas.051631398}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Pöhlmann, Stefan and Soilleux, Elizabeth J. and Baribaud, Frédéric and Leslie, George J. and Morris, Lesley S. and Trowsdale, John and Lee, Benhur and Coleman, Nicholas and Doms, Robert W.}, year={2001}, month=feb, pages={2670–2675} }

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  "abstract": "<jats:p>DC-SIGN, a C-type lectin expressed on the surface of dendritic cells (DCs), efficiently binds and transmits HIVs and simian immunodeficiency viruses to susceptible cells in trans. A DC-SIGN homologue, termed DC-SIGNR, has recently been described. Herein we show that DC-SIGNR, like DC-SIGN, can bind to multiple strains of HIV-1, HIV-2, and simian immunodeficiency virus and transmit these viruses to both T cell lines and human peripheral blood mononuclear cells. Binding of virus to DC-SIGNR was dependent on carbohydrate recognition. Immunostaining with a DC-SIGNR-specific antiserum showed that DC-SIGNR was expressed on sinusoidal endothelial cells in the liver and on endothelial cells in lymph node sinuses and placental villi. The presence of this efficient virus attachment factor on multiple endothelial cell types indicates that DC-SIGNR could play a role in the vertical transmission of primate lentiviruses, in the enabling of HIV to traverse the capillary endothelium in some organs, and in the presentation of virus to CD4-positive cells in multiple locations including lymph nodes.</jats:p>",
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