Subcellular localization of acetyl-CoA carboxylase in the apicomplexan parasite Toxoplasma gondii
10.1073/pnas.051629998
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

Apicomplexan parasites such as Toxoplasma gondii contain a primitive plastid, the apicoplast, whose genome consists of a 35-kb circular DNA related to the plastid DNA of plants. Plants synthesize fatty acids in their plastids. The first committed step in fatty acid synthesis is catalyzed by acetyl-CoA carboxylase (ACC). This enzyme is encoded in the nucleus, synthesized in the cytosol, and transported into the plastid. In the present work, two genes encoding ACC from T. gondii were cloned and the gene structure was determined. Both ORFs encode multidomain proteins, each with an N-terminal extension, compared with the cytosolic ACCs from plants. The N-terminal extension of one isozyme, ACC1, was shown to target green fluorescent protein to the apicoplast of T. gondii . In addition, the apicoplast contains a biotinylated protein, consistent with the assertion that ACC1 is localized there. The second ACC in T. gondii appears to be cytosolic. T. gondii mitochondria also contain a biotinylated protein, probably pyruvate carboxylase. These results confirm the essential nature of the apicoplast and explain the inhibition of parasite growth in cultured cells by herbicides targeting ACC.

Bibliography

Jelenska, J., Crawford, M. J., Harb, O. S., Zuther, E., Haselkorn, R., Roos, D. S., & Gornicki, P. (2001). Subcellular localization of acetyl-CoA carboxylase in the apicomplexan parasite Toxoplasma gondii. Proceedings of the National Academy of Sciences, 98(5), 2723–2728.

Authors 7 University of Pennsylvania
  1. J. Jelenska (first) University of Pennsylvania
  2. M. J. Crawford (additional) University of Pennsylvania
  3. O. S. Harb (additional) University of Pennsylvania
  4. E. Zuther (additional) University of Pennsylvania
  5. R. Haselkorn (additional) University of Pennsylvania
  6. D. S. Roos (additional) University of Pennsylvania
  7. P. Gornicki (additional) University of Pennsylvania
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Dates
Type When
Created 23 years, 1 month ago (July 26, 2002, 10:45 a.m.)
Deposited 3 years, 2 months ago (June 7, 2022, 1:28 a.m.)
Indexed 1 month, 2 weeks ago (July 14, 2025, 11:36 p.m.)
Issued 24 years, 6 months ago (Feb. 13, 2001)
Published 24 years, 6 months ago (Feb. 13, 2001)
Published Online 24 years, 6 months ago (Feb. 13, 2001)
Published Print 24 years, 6 months ago (Feb. 27, 2001)
Funders 0

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@article{Jelenska_2001, title={Subcellular localization of acetyl-CoA carboxylase in the apicomplexan parasite Toxoplasma gondii}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.051629998}, DOI={10.1073/pnas.051629998}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Jelenska, J. and Crawford, M. J. and Harb, O. S. and Zuther, E. and Haselkorn, R. and Roos, D. S. and Gornicki, P.}, year={2001}, month=feb, pages={2723–2728} }