DOI: 10.1073/pnas.051624498. Altering dimerization specificity by changes in surface electrostatics

Altering dimerization specificity by changes in surface electrostatics

10.1073/pnas.051624498

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Arc repressor forms a homodimer in which the subunits intertwine to create a single globular domain. To obtain Arc sequences that fold preferentially as heterodimers, variants with surface patches of excess positive or negative charge were designed. Several but not all oppositely charged sequence pairs showed preferential heterodimer formation. In the most successful design pair, α helix B of one subunit contained glutamic acids at positions 43, 46, 47, 48, and 50, whereas the other subunit contained lysines or arginines at these positions. A continuum electrostatic model captures many features of the experimental results and suggests that the most successful designs include elements of both positive and negative design.

Bibliography

Nohaile, M. J., Hendsch, Z. S., Tidor, B., & Sauer, R. T. (2001). Altering dimerization specificity by changes in surface electrostatics. Proceedings of the National Academy of Sciences, 98(6), 3109â3114.

Authors 4

M. J. Nohaile (first)
Z. S. Hendsch (additional)
B. Tidor (additional)
R. T. Sauer (additional)

References 29 Referenced 20

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:41 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 3:16 p.m.)
Indexed	1 year ago (Aug. 19, 2024, 5:48 a.m.)
Issued	24 years, 6 months ago (Feb. 27, 2001)
Published	24 years, 6 months ago (Feb. 27, 2001)
Published Online	24 years, 6 months ago (Feb. 27, 2001)
Published Print	24 years, 5 months ago (March 13, 2001)

Funders 0

None

BibTeX

@article{Nohaile_2001, title={Altering dimerization specificity by changes in surface electrostatics}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.051624498}, DOI={10.1073/pnas.051624498}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Nohaile, M. J. and Hendsch, Z. S. and Tidor, B. and Sauer, R. T.}, year={2001}, month=feb, pages={3109–3114} }

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