DOI: 10.1073/pnas.051454398. Selenoprotein oxidoreductase with specificity for thioredoxin and glutathione systems

Selenoprotein oxidoreductase with specificity for thioredoxin and glutathione systems

10.1073/pnas.051454398

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Thioredoxin (Trx) and glutathione (GSH) systems are considered to be two major redox systems in animal cells. They are reduced by NADPH via Trx reductase (TR) or oxidized GSH (GSSG) reductase and further supply electrons for deoxyribonucleotide synthesis, antioxidant defense, and redox regulation of signal transduction, transcription, cell growth, and apoptosis. We cloned and characterized a pyridine nucleotide disulfide oxidoreductase, Trx and GSSG reductase (TGR), that exhibits specificity for both redox systems. This enzyme contains a selenocysteine residue encoded by the TGA codon. TGR can reduce Trx, GSSG, and a GSH-linked disulfide in in vitro assays. This unusual substrate specificity is achieved by an evolutionary conserved fusion of the TR and glutaredoxin domains. These observations, together with the biochemical probing and molecular modeling of the TGR structure, suggest a mechanism whereby the C-terminal selenotetrapeptide serves a role of a protein-linked GSSG and shuttles electrons from the disulfide center within the TR domain to either the glutaredoxin domain or Trx.

Bibliography

Sun, Q.-A., Kirnarsky, L., Sherman, S., & Gladyshev, V. N. (2001). Selenoprotein oxidoreductase with specificity for thioredoxin and glutathione systems. Proceedings of the National Academy of Sciences, 98(7), 3673â3678.

Authors 4

Qi-An Sun (first)
Leo Kirnarsky (additional)
Simon Sherman (additional)
Vadim N. Gladyshev (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:44 a.m.)
Deposited	3 years, 3 months ago (June 7, 2022, 1:43 a.m.)
Indexed	2 weeks, 4 days ago (Aug. 19, 2025, 6:19 a.m.)
Issued	24 years, 5 months ago (March 20, 2001)
Published	24 years, 5 months ago (March 20, 2001)
Published Online	24 years, 5 months ago (March 20, 2001)
Published Print	24 years, 5 months ago (March 27, 2001)

Funders 0

None

BibTeX

@article{Sun_2001, title={Selenoprotein oxidoreductase with specificity for thioredoxin and glutathione systems}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.051454398}, DOI={10.1073/pnas.051454398}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sun, Qi-An and Kirnarsky, Leo and Sherman, Simon and Gladyshev, Vadim N.}, year={2001}, month=mar, pages={3673–3678} }

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