DOI: 10.1073/pnas.0510305103. A structural role for ATP in the formation and stability of the human origin recognition complex

A structural role for ATP in the formation and stability of the human origin recognition complex

10.1073/pnas.0510305103

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The locally restricted recruitment of the multisubunit origin recognition complex (ORC) to eukaryotic chromosomes defines the position of origins of DNA replication. In budding yeast and metazoans the DNA binding activity of ORC is stimulated by ATP and requires an AAA+-type nucleotide binding domain in the largest subunit. Little else is known about the mechanisms behind the ATP requirement for ORC in its initiator function and, specifically, the relevance of nucleotide binding domains present on other subunits. Here we show that ATP is required for specific subunit interactions in the human ORC, with the Orc4 subunit playing a critical role in this dynamic process. ATP is essential for the maintenance of ORC integrity and facilitates complex formation. Thus, besides its previously identified role in DNA binding, ATP serves also as a structural cofactor for human ORC.

Bibliography

Ranjan, A., & Gossen, M. (2006). A structural role for ATP in the formation and stability of the human origin recognition complex. Proceedings of the National Academy of Sciences, 103(13), 4864â4869.

Authors 2

Anand Ranjan (first)
Manfred Gossen (additional)

References 33 Referenced 42

Dates

Type	When
Created	19 years, 5 months ago (March 20, 2006, 8:44 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 10:20 a.m.)
Indexed	4 months ago (April 17, 2025, 3:27 p.m.)
Issued	19 years, 5 months ago (March 20, 2006)
Published	19 years, 5 months ago (March 20, 2006)
Published Online	19 years, 5 months ago (March 20, 2006)
Published Print	19 years, 4 months ago (March 28, 2006)

Funders 0

None

BibTeX

@article{Ranjan_2006, title={A structural role for ATP in the formation and stability of the human origin recognition complex}, volume={103}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0510305103}, DOI={10.1073/pnas.0510305103}, number={13}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Ranjan, Anand and Gossen, Manfred}, year={2006}, month=mar, pages={4864–4869} }

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