DOI: 10.1073/pnas.0508491103. Odorant responses of olfactory sensory neurons expressing the odorant receptor MOR23: A patch clamp analysis in gene-targeted mice [Penn]

Odorant responses of olfactory sensory neurons expressing the odorant receptor MOR23: A patch clamp analysis in gene-targeted mice

10.1073/pnas.0508491103

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A glomerulus in the mammalian olfactory bulb receives axonal inputs from olfactory sensory neurons (OSNs) that express the same odorant receptor (OR). Glomeruli are generally thought to represent functional units of olfactory coding, but there are no data on the electrophysiological properties of OSNs that express the same endogenous OR. Here, using patch clamp recordings in an intact epithelial preparation, we directly measured the transduction currents and receptor potentials from the dendritic knobs of mouse OSNs that express the odorant receptor MOR23 along with the green fluorescent protein. All of the 53 cells examined responded to lyral, a known ligand for MOR23. There were profound differences in response kinetics, particularly in the deactivation phase. The cells were very sensitive to lyral, with some cells responding to as little as 10 nM. The dynamic range was unexpectedly broad, with threshold and saturation in individual cells often covering three log units of lyral concentration. The potential causes and biological significance of this cellular heterogeneity are discussed. Patch clamp recording from OSNs that express a defined OR provides a powerful approach to investigate the sensory inputs to individual glomeruli.

Bibliography

Grosmaitre, X., Vassalli, A., Mombaerts, P., Shepherd, G. M., & Ma, M. (2006). Odorant responses of olfactory sensory neurons expressing the odorant receptor MOR23: A patch clamp analysis in gene-targeted mice. Proceedings of the National Academy of Sciences, 103(6), 1970â1975.

Authors 5

Xavier Grosmaitre (first)
Anne Vassalli (additional)
Peter Mombaerts (additional)
Gordon M. Shepherd (additional)
Minghong Ma (additional)

References 46 Referenced 132

Dates

Type	When
Created	19 years, 6 months ago (Jan. 30, 2006, 9:03 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 12:37 p.m.)
Indexed	1 month ago (July 26, 2025, 5:32 a.m.)
Issued	19 years, 7 months ago (Jan. 30, 2006)
Published	19 years, 7 months ago (Jan. 30, 2006)
Published Online	19 years, 7 months ago (Jan. 30, 2006)
Published Print	19 years, 6 months ago (Feb. 7, 2006)

Funders 0

None

BibTeX

@article{Grosmaitre_2006, title={Odorant responses of olfactory sensory neurons expressing the odorant receptor MOR23: A patch clamp analysis in gene-targeted mice}, volume={103}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0508491103}, DOI={10.1073/pnas.0508491103}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Grosmaitre, Xavier and Vassalli, Anne and Mombaerts, Peter and Shepherd, Gordon M. and Ma, Minghong}, year={2006}, month=jan, pages={1970–1975} }

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