DOI: 10.1073/pnas.0507436103. Consequences of the selective blockage of chaperone-mediated autophagy

Consequences of the selective blockage of chaperone-mediated autophagy

10.1073/pnas.0507436103

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Chaperone-mediated autophagy (CMA) is a selective pathway for the degradation of cytosolic proteins in lysosomes. CMA declines with age because of a decrease in the levels of lysosome-associated membrane protein (LAMP) type 2A, a lysosomal receptor for this pathway. We have selectively blocked the expression of LAMP-2A in mouse fibroblasts in culture and analyzed the cellular consequences of reduced CMA activity. CMA-defective cells maintain normal rates of long-lived protein degradation by up-regulating macroautophagy, the major form of autophagy. Constitutive up-regulation of macroautophagy is unable, however, to compensate for all CMA functions. Thus, CMA-defective cells are more sensitive to stressors, suggesting that, although protein turnover is maintained, the selectivity of CMA is necessary as part of the cellular response to stress. Our results also denote the existence of cross-talk among different forms of autophagy.

Bibliography

Massey, A. C., Kaushik, S., Sovak, G., Kiffin, R., & Cuervo, A. M. (2006). Consequences of the selective blockage of chaperone-mediated autophagy. Proceedings of the National Academy of Sciences, 103(15), 5805â5810.

Authors 5

Ashish C. Massey (first)
Susmita Kaushik (additional)
Guy Sovak (additional)
Roberta Kiffin (additional)
Ana Maria Cuervo (additional)

References 30 Referenced 432

Dates

Type	When
Created	19 years, 4 months ago (April 3, 2006, 8:35 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 10:06 a.m.)
Indexed	2 days, 12 hours ago (Aug. 27, 2025, 12:36 p.m.)
Issued	19 years, 4 months ago (April 11, 2006)
Published	19 years, 4 months ago (April 11, 2006)
Published Online	19 years, 4 months ago (April 11, 2006)
Published Print	19 years, 4 months ago (April 11, 2006)

Funders 0

None

BibTeX

@article{Massey_2006, title={Consequences of the selective blockage of chaperone-mediated autophagy}, volume={103}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0507436103}, DOI={10.1073/pnas.0507436103}, number={15}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Massey, Ashish C. and Kaushik, Susmita and Sovak, Guy and Kiffin, Roberta and Cuervo, Ana Maria}, year={2006}, month=apr, pages={5805–5810} }

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