DOI: 10.1073/pnas.0507250102. Long-term renewal of hair follicles from clonogenic multipotent stem cells

Long-term renewal of hair follicles from clonogenic multipotent stem cells

10.1073/pnas.0507250102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Adult stem cells are essential for tissue renewal, regeneration, and repair, and their expansion in culture is of paramount importance for regenerative medicine. Using the whisker follicle of the rat as a model system, we demonstrate that (i) clonogenicity is an intrinsic property of the adult stem cells of the hair follicle; (ii) after cultivation for >140 doublings, these stem cells, transplanted to the dermo-epidermal junction of newborn mouse skin, form part or all of the developing follicles; (iii) the stem cells incorporated into follicles are multipotent, because they generate all of the lineages of the hair follicle and sebaceous gland; (iv) thousands of hair follicles can be generated from the progeny of a single cultivated stem cell; (v) cultured stem cells express the self-renewal genesBmi1andZfp145;(vi) several stem cells participate in the formation of a single hair bulb; and (vii) there are many more stem cells in whisker follicles than could be anticipated from label-retaining experiments.

Bibliography

Claudinot, S., Nicolas, M., Oshima, H., Rochat, A., & Barrandon, Y. (2005). Long-term renewal of hair follicles from clonogenic multipotent stem cells. Proceedings of the National Academy of Sciences, 102(41), 14677â14682.

Authors 5

Stéphanie Claudinot (first)
Michael Nicolas (additional)
Hideo Oshima (additional)
Ariane Rochat (additional)
Yann Barrandon (additional)

References 44 Referenced 260

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Rochat, A. & Barrandon, Y. (2004) in Handbook of Stem Cells: Adult and Fetal Stem Cells, ed. Lanza, R. (Elsevier, Amsterdam), Vol. 2, pp. 763-772. / Handbook of Stem Cells: Adult and Fetal Stem Cells (2004)
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Dates

Type	When
Created	19 years, 11 months ago (Oct. 3, 2005, 8:33 p.m.)
Deposited	1 year, 7 months ago (Jan. 30, 2024, 7:49 a.m.)
Indexed	5 days, 14 hours ago (Aug. 30, 2025, 1:04 p.m.)
Issued	19 years, 11 months ago (Oct. 3, 2005)
Published	19 years, 11 months ago (Oct. 3, 2005)
Published Online	19 years, 11 months ago (Oct. 3, 2005)
Published Print	19 years, 10 months ago (Oct. 11, 2005)

Funders 0

None

BibTeX

@article{Claudinot_2005, title={Long-term renewal of hair follicles from clonogenic multipotent stem cells}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0507250102}, DOI={10.1073/pnas.0507250102}, number={41}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Claudinot, Stéphanie and Nicolas, Michael and Oshima, Hideo and Rochat, Ariane and Barrandon, Yann}, year={2005}, month=oct, pages={14677–14682} }

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