DOI: 10.1073/pnas.0506961102. An allosteric model for heterogeneous receptor complexes: Understanding bacterial chemotaxis responses to multiple stimuli

An allosteric model for heterogeneous receptor complexes: Understanding bacterial chemotaxis responses to multiple stimuli

10.1073/pnas.0506961102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The classical Monod-Wyman-Changeux model for homogeneous allosteric protein complex is generalized in this article to model the responses of heterogeneous receptor complexes to multiple types of ligand stimulus. We show that the recent in vivo experimental data of Escherichia coli chemotaxis responses for mutant strains with different expression levels of the chemo-receptors to different types of stimulus [Sourjik, V. & Berg, H. C. (2004) Nature 428, 437–441] all can be explained consistently within this generalized Monod-Wyman-Changeux model. Based on the model and the existing data, responses of all of the strains (studied in this article) to the presence of any combinations of ligand (Ser and MeAsp) concentrations are predicted quantitatively for future experimental verification. Through modeling the in vivo response data, our study reveals important information about the properties of different types of individual receptors, as well as the composition of the cluster. The energetic contribution of the nonligand binding, cytoplasmic parts of the cluster, such as CheA and CheW, is also discussed. The generalized allosteric model provides a consistent framework in understanding signal integration and differentiation in bacterial chemotaxis. It should also be useful for studying the functions of other heterogeneous receptor complexes.

Bibliography

Mello, B. A., & Tu, Y. (2005). An allosteric model for heterogeneous receptor complexes: Understanding bacterial chemotaxis responses to multiple stimuli. Proceedings of the National Academy of Sciences, 102(48), 17354â17359.

Authors 2

Bernardo A. Mello (first)
Yuhai Tu (additional)

References 26 Referenced 139

Dates

Type	When
Created	19 years, 9 months ago (Nov. 17, 2005, 8:33 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 10:12 a.m.)
Indexed	2 months, 2 weeks ago (June 4, 2025, 6:29 a.m.)
Issued	19 years, 9 months ago (Nov. 17, 2005)
Published	19 years, 9 months ago (Nov. 17, 2005)
Published Online	19 years, 9 months ago (Nov. 17, 2005)
Published Print	19 years, 8 months ago (Nov. 29, 2005)

Funders 0

None

BibTeX

@article{Mello_2005, title={An allosteric model for heterogeneous receptor complexes: Understanding bacterial chemotaxis responses to multiple stimuli}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0506961102}, DOI={10.1073/pnas.0506961102}, number={48}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Mello, Bernardo A. and Tu, Yuhai}, year={2005}, month=nov, pages={17354–17359} }

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