DOI: 10.1073/pnas.0506109102. Molecular-level secondary structure, polymorphism, and dynamics of full-length α-synuclein fibrils studied by solid-state NMR

Molecular-level secondary structure, polymorphism, and dynamics of full-length α-synuclein fibrils studied by solid-state NMR

10.1073/pnas.0506109102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The 140-residue protein α-synuclein (AS) is able to form amyloid fibrils and as such is the main component of protein inclusions involved in Parkinson's disease. We have investigated the structure and dynamics of full-length AS fibrils by high-resolution solid-state NMR spectroscopy. Homonuclear and heteronuclear 2D and 3D spectra of fibrils grown from uniformly 13 C/ 15 N-labeled AS and AS reverse-labeled for two of the most abundant amino acids, K and V, were analyzed. 13 C and 15 N signals exhibited linewidths of <0.7 ppm. Sequential assignments were obtained for 48 residues in the hydrophobic core region. We identified two different types of fibrils displaying chemical-shift differences of up to 13 ppm in the 15 N dimension and up to 5 ppm for backbone and side-chain 13 C chemical shifts. EM studies suggested that molecular structure is correlated with fibril morphology. Investigation of the secondary structure revealed that most amino acids of the core region belong to β-strands with similar torsion angles in both conformations. Selection of regions with different mobility indicated the existence of monomers in the sample and allowed the identification of mobile segments of the protein within the fibril in the presence of monomeric protein. At least 35 C-terminal residues were mobile and lacked a defined secondary structure, whereas the N terminus was rigid starting from residue 22. Our findings agree well with the overall picture obtained with other methods and provide insight into the amyloid fibril structure and dynamics with residue-specific resolution.

Bibliography

Heise, H., Hoyer, W., Becker, S., Andronesi, O. C., Riedel, D., & Baldus, M. (2005). Molecular-level secondary structure, polymorphism, and dynamics of full-length Î±-synuclein fibrils studied by solid-state NMR. Proceedings of the National Academy of Sciences, 102(44), 15871â15876.

Authors 6

Henrike Heise (first)
Wolfgang Hoyer (additional)
Stefan Becker (additional)
Ovidiu C. Andronesi (additional)
Dietmar Riedel (additional)
Marc Baldus (additional)

References 56 Referenced 582

Dates

Type	When
Created	19 years, 10 months ago (Oct. 26, 2005, 4:43 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 1:37 p.m.)
Indexed	1 day, 23 hours ago (Sept. 4, 2025, 9:53 a.m.)
Issued	19 years, 10 months ago (Oct. 24, 2005)
Published	19 years, 10 months ago (Oct. 24, 2005)
Published Online	19 years, 10 months ago (Oct. 24, 2005)
Published Print	19 years, 10 months ago (Nov. 1, 2005)

Funders 0

None

BibTeX

@article{Heise_2005, title={Molecular-level secondary structure, polymorphism, and dynamics of full-length α-synuclein fibrils studied by solid-state NMR}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0506109102}, DOI={10.1073/pnas.0506109102}, number={44}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Heise, Henrike and Hoyer, Wolfgang and Becker, Stefan and Andronesi, Ovidiu C. and Riedel, Dietmar and Baldus, Marc}, year={2005}, month=oct, pages={15871–15876} }

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