DOI: 10.1073/pnas.050586197. Identification of <i>CDK4</i> as a target of c-MYC

Identification of CDK4 as a target of c-MYC

10.1073/pnas.050586197

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The prototypic oncogene c- MYC encodes a transcription factor that can drive proliferation by promoting cell-cycle reentry. However, the mechanisms through which c-MYC achieves these effects have been unclear. Using serial analysis of gene expression, we have identified the cyclin-dependent kinase 4 ( CDK4 ) gene as a transcriptional target of c-MYC. c-MYC induced a rapid increase in CDK4 mRNA levels through four highly conserved c-MYC binding sites within the CDK4 promoter. Cell-cycle progression is delayed in c- MYC -deficient RAT1 cells, and this delay was associated with a defect in CDK4 induction. Ectopic expression of CDK4 in these cells partially alleviated the growth defect. Thus, CDK4 provides a direct link between the oncogenic effects of c- MYC and cell-cycle regulation.

Bibliography

Hermeking, H., Rago, C., Schuhmacher, M., Li, Q., Barrett, J. F., Obaya, A. J., OâConnell, B. C., Mateyak, M. K., Tam, W., Kohlhuber, F., Dang, C. V., Sedivy, J. M., Eick, D., Vogelstein, B., & Kinzler, K. W. (2000). Identification of CDK4 as a target of c-MYC. Proceedings of the National Academy of Sciences, 97(5), 2229â2234.

Authors 15

Heiko Hermeking (first)
Carlo Rago (additional)
Marino Schuhmacher (additional)
Qing Li (additional)
John F. Barrett (additional)
Alvaro J. Obaya (additional)
Brenda C. O'Connell (additional)
Maria K. Mateyak (additional)
Wanny Tam (additional)
Franz Kohlhuber (additional)
Chi V. Dang (additional)
John M. Sedivy (additional)
Dirk Eick (additional)
Bert Vogelstein (additional)
Kenneth W. Kinzler (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 2 months ago (June 7, 2022, 1:10 a.m.)
Indexed	1 week, 1 day ago (Aug. 22, 2025, 12:45 a.m.)
Issued	25 years, 6 months ago (Feb. 25, 2000)
Published	25 years, 6 months ago (Feb. 25, 2000)
Published Online	25 years, 6 months ago (Feb. 25, 2000)
Published Print	25 years, 6 months ago (Feb. 29, 2000)

Funders 0

None

BibTeX

@article{Hermeking_2000, title={Identification of CDK4 as a target of c-MYC}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.050586197}, DOI={10.1073/pnas.050586197}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hermeking, Heiko and Rago, Carlo and Schuhmacher, Marino and Li, Qing and Barrett, John F. and Obaya, Alvaro J. and O’Connell, Brenda C. and Mateyak, Maria K. and Tam, Wanny and Kohlhuber, Franz and Dang, Chi V. and Sedivy, John M. and Eick, Dirk and Vogelstein, Bert and Kinzler, Kenneth W.}, year={2000}, month=feb, pages={2229–2234} }

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