DOI: 10.1073/pnas.0505581102. Structure of trigger factor binding domain in biologically homologous complex with eubacterial ribosome reveals its chaperone action

Structure of trigger factor binding domain in biologically homologous complex with eubacterial ribosome reveals its chaperone action

10.1073/pnas.0505581102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Trigger factor (TF), the first chaperone in eubacteria to encounter the emerging nascent chain, binds to the large ribosomal subunit in the vicinity of the protein exit tunnel opening and forms a sheltered folding space. Here, we present the 3.5-Å crystal structure of the physiological complex of the large ribosomal subunit from the eubacterium Deinococcus radiodurans with the N-terminal domain of TF (TFa) from the same organism. For anchoring, TFa exploits a small ribosomal surface area in the vicinity of proteins L23 and L29, by using its “signature motif” as well as additional structural elements. The molecular details of TFa interactions reveal that L23 is essential for the association of TF with the ribosome and may serve as a channel of communication with the nascent chain progressing in the tunnel. L29 appears to induce a conformational change in TFa, which results in the exposure of TFa hydrophobic patches to the opening of the ribosomal exit tunnel, thus increasing its affinity for hydrophobic segments of the emerging nascent polypeptide. This observation implies that, in addition to creating a protected folding space for the emerging nascent chain, TF association with the ribosome prevents aggregation by providing a competing hydrophobic environment and may be critical for attaining the functional conformation necessary for chaperone activity.

Bibliography

Baram, D., Pyetan, E., Sittner, A., Auerbach-Nevo, T., Bashan, A., & Yonath, A. (2005). Structure of trigger factor binding domain in biologically homologous complex with eubacterial ribosome reveals its chaperone action. Proceedings of the National Academy of Sciences, 102(34), 12017â12022.

Authors 6

David Baram (first)
Erez Pyetan (additional)
Assa Sittner (additional)
Tamar Auerbach-Nevo (additional)
Anat Bashan (additional)
Ada Yonath (additional)

References 32 Referenced 96

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Dates

Type	When
Created	20 years ago (Aug. 9, 2005, 10:53 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 2:19 p.m.)
Indexed	1 week, 2 days ago (Aug. 12, 2025, 5:33 p.m.)
Issued	20 years ago (Aug. 9, 2005)
Published	20 years ago (Aug. 9, 2005)
Published Online	20 years ago (Aug. 9, 2005)
Published Print	19 years, 11 months ago (Aug. 23, 2005)

Funders 0

None

BibTeX

@article{Baram_2005, title={Structure of trigger factor binding domain in biologically homologous complex with eubacterial ribosome reveals its chaperone action}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0505581102}, DOI={10.1073/pnas.0505581102}, number={34}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Baram, David and Pyetan, Erez and Sittner, Assa and Auerbach-Nevo, Tamar and Bashan, Anat and Yonath, Ada}, year={2005}, month=aug, pages={12017–12022} }

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  "abstract": "<jats:p>\n            Trigger factor (TF), the first chaperone in eubacteria to encounter the emerging nascent chain, binds to the large ribosomal subunit in the vicinity of the protein exit tunnel opening and forms a sheltered folding space. Here, we present the 3.5-\u00c5 crystal structure of the physiological complex of the large ribosomal subunit from the eubacterium\n            <jats:italic>Deinococcus radiodurans</jats:italic>\n            with the N-terminal domain of TF (TFa) from the same organism. For anchoring, TFa exploits a small ribosomal surface area in the vicinity of proteins L23 and L29, by using its \u201csignature motif\u201d as well as additional structural elements. The molecular details of TFa interactions reveal that L23 is essential for the association of TF with the ribosome and may serve as a channel of communication with the nascent chain progressing in the tunnel. L29 appears to induce a conformational change in TFa, which results in the exposure of TFa hydrophobic patches to the opening of the ribosomal exit tunnel, thus increasing its affinity for hydrophobic segments of the emerging nascent polypeptide. This observation implies that, in addition to creating a protected folding space for the emerging nascent chain, TF association with the ribosome prevents aggregation by providing a competing hydrophobic environment and may be critical for attaining the functional conformation necessary for chaperone activity.\n          </jats:p>",
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