DOI: 10.1073/pnas.0505337102. Association of the human papillomavirus type 16 E7 oncoprotein with the 600-kDa retinoblastoma protein-associated factor, p600

Association of the human papillomavirus type 16 E7 oncoprotein with the 600-kDa retinoblastoma protein-associated factor, p600

10.1073/pnas.0505337102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The human papillomavirus type 16 (HPV-16) E7 gene encodes a multifunctional oncoprotein that can subvert multiple cellular regulatory pathways. The best-known cellular targets of the HPV-16 E7 oncoprotein are the retinoblastoma tumor suppressor protein pRB and the related pocket proteins p107 and p130. However, there is ample evidence that E7 has additional cellular targets that contribute to its transforming potential. We isolated HPV-16 E7 associated cellular protein complexes by tandem affinity purification and mass spectrometry and identified the 600-kDa retinoblastoma protein associated factor, p600, as a cellular target of E7. Association of E7 with p600 is independent of the pocket proteins and is mediated through the N terminal E7 domain, which is related to conserved region 1 of the adenovirus E1A protein and importantly contributes to cellular transformation independent of pRB binding. Depletion of p600 protein levels by RNA interference substantially decreased anchorage-independent growth in HPV-positive and -negative human cancer cells. Therefore, p600 is a cellular target of E7 that regulates cellular pathways that contribute to anchorage-independent growth and cellular transformation.

Bibliography

Huh, K.-W., DeMasi, J., Ogawa, H., Nakatani, Y., Howley, P. M., & MÃ¼nger, K. (2005). Association of the human papillomavirus type 16 E7 oncoprotein with the 600-kDa retinoblastoma protein-associated factor, p600. Proceedings of the National Academy of Sciences, 102(32), 11492â11497.

Authors 6

Kyung-Won Huh (first)
Joseph DeMasi (additional)
Hidesato Ogawa (additional)
Yoshihiro Nakatani (additional)
Peter M. Howley (additional)
Karl Münger (additional)

References 42 Referenced 157

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Dates

Type	When
Created	20 years, 1 month ago (Aug. 2, 2005, 3:45 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 10:08 a.m.)
Indexed	2 months ago (July 2, 2025, 5:18 a.m.)
Issued	20 years, 1 month ago (Aug. 1, 2005)
Published	20 years, 1 month ago (Aug. 1, 2005)
Published Online	20 years, 1 month ago (Aug. 1, 2005)
Published Print	20 years ago (Aug. 9, 2005)

Funders 0

None

BibTeX

@article{Huh_2005, title={Association of the human papillomavirus type 16 E7 oncoprotein with the 600-kDa retinoblastoma protein-associated factor, p600}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0505337102}, DOI={10.1073/pnas.0505337102}, number={32}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Huh, Kyung-Won and DeMasi, Joseph and Ogawa, Hidesato and Nakatani, Yoshihiro and Howley, Peter M. and Münger, Karl}, year={2005}, month=aug, pages={11492–11497} }

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