DOI: 10.1073/pnas.0504995102. Functional conformational motions in the turnover cycle of cholesterol oxidase

Functional conformational motions in the turnover cycle of cholesterol oxidase

10.1073/pnas.0504995102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Reexamining experimental data of single-molecule fluorescence correlation spectroscopy for cholesterol oxidase, we find that the existing Michaelis–Menten models with dynamical disorder cannot explain strong correlations between subsequent turnover cycles revealed in the diagonal feature in the joint statistical distribution of adjacent “on” times of this enzyme. We suggest that functional conformational motions representing ordered sequences of transitions between a set of conformational substates are involved, along with equilibrium conformational fluctuations in the turnover cycle of cholesterol oxidase. A two-channel model of single-enzyme dynamics, including a slow functional conformational motion in one of the channels, is proposed that allows us to reproduce such strong correlations.

Bibliography

Lerch, H.-P., Rigler, R., & Mikhailov, A. S. (2005). Functional conformational motions in the turnover cycle of cholesterol oxidase. Proceedings of the National Academy of Sciences, 102(31), 10807â10812.

Authors 3

Hans-Philipp Lerch (first)
Rudolf Rigler (additional)
Alexander S. Mikhailov (additional)

References 29 Referenced 57

Dates

Type	When
Created	20 years, 1 month ago (July 26, 2005, 10:43 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 2:13 p.m.)
Indexed	5 months ago (March 26, 2025, 1:41 p.m.)
Issued	20 years, 1 month ago (July 26, 2005)
Published	20 years, 1 month ago (July 26, 2005)
Published Online	20 years, 1 month ago (July 26, 2005)
Published Print	20 years ago (Aug. 2, 2005)

Funders 0

None

BibTeX

@article{Lerch_2005, title={Functional conformational motions in the turnover cycle of cholesterol oxidase}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0504995102}, DOI={10.1073/pnas.0504995102}, number={31}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Lerch, Hans-Philipp and Rigler, Rudolf and Mikhailov, Alexander S.}, year={2005}, month=jul, pages={10807–10812} }

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