DOI: 10.1073/pnas.0504235102. Stable barley chromosomes without centromeric repeats

Stable barley chromosomes without centromeric repeats

10.1073/pnas.0504235102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The satellite sequences (AGGGAG) n and Ty 3 / gypsy -like retrotransposons are known to localize at the barley centromeres. Using a gametocidal system, which induces chromosomal mutations in barley chromosomes added to common wheat, we obtained an isochromosome for the short arm of barley chromosome 7H (7HS) that lacked the barley-specific satellite sequence (AGGGAG) n . Two telocentric derivatives of the isochromosome arose in the progeny: 7HS* with and 7HS** without the pericentromeric C-band. FISH analysis demonstrated that both telosomes lacked not only the barley-specific centromeric (AGGGAG) n repeats and retroelements but also any of the known wheat centromeric tandem repeats, including the 192-bp, 250-bp, and TaiI sequences. Although they lacked these centromeric repeats, 7HS* and 7HS** both showed normal mitotic and meiotic transmission. Translocation of barley centromeric repeats to a wheat chromosome 4A did not generate a dicentric chromosome. Indirect immunostaining revealed that all tested centromere-specific proteins (rice CENH3, maize CENP-C, and putative barley homologues of the yeast kinetochore proteins CBF5 and SKP1) and histone H3 phosphorylated at serines 10 and 28 localized at the centromeric region of 7HS*. We conclude that the barley centromeric repeats are neither sufficient nor obligatory to assemble kinetochores, and we discuss the possible formation of a novel centromere in a barley chromosome.

Bibliography

Nasuda, S., Hudakova, S., Schubert, I., Houben, A., & Endo, T. R. (2005). Stable barley chromosomes without centromeric repeats. Proceedings of the National Academy of Sciences, 102(28), 9842â9847.

Authors 5

S. Nasuda (first)
S. Hudakova (additional)
I. Schubert (additional)
A. Houben (additional)
T. R. Endo (additional)

References 49 Referenced 172

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Dates

Type	When
Created	20 years, 2 months ago (July 5, 2005, 11:23 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 2:18 p.m.)
Indexed	1 month, 1 week ago (July 26, 2025, 4:44 a.m.)
Issued	20 years, 2 months ago (July 5, 2005)
Published	20 years, 2 months ago (July 5, 2005)
Published Online	20 years, 2 months ago (July 5, 2005)
Published Print	20 years, 1 month ago (July 12, 2005)

Funders 0

None

BibTeX

@article{Nasuda_2005, title={Stable barley chromosomes without centromeric repeats}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0504235102}, DOI={10.1073/pnas.0504235102}, number={28}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Nasuda, S. and Hudakova, S. and Schubert, I. and Houben, A. and Endo, T. R.}, year={2005}, month=jul, pages={9842–9847} }

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