DOI: 10.1073/pnas.0503968102. Fractal intermediates in the self-assembly of silicatein filaments

Fractal intermediates in the self-assembly of silicatein filaments

10.1073/pnas.0503968102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Silicateins are proteins with catalytic, structure-directing activity that are responsible for silica biosynthesis in certain sponges; they are the constituents of macroscopic protein filaments that are found occluded within the silica needles made by Tethya aurantia . Self-assembly of the silicatein monomers and oligomers is shown to form fibrous structures by a mechanism that is fundamentally different from any previously described filament-assembly process. This assembly proceeds through the formation of diffusion-limited, fractally patterned aggregates on the path to filament formation. The driving force for this self-assembly is suggested to be entropic, mediated by the interaction of hydrophobic patches on the surfaces of the silicatein subunits that are not found on highly homologous congeners that do not form filaments. Our results are consistent with a model in which silicatein monomers associate into oligomers that are stabilized by intermolecular disulfide bonds. These oligomeric units assemble into a fractal network that subsequently condenses and organizes into a filamentous structure. These results represent a potentially general mechanism for protein fiber self-assembly.

Bibliography

Murr, M. M., & Morse, D. E. (2005). Fractal intermediates in the self-assembly of silicatein filaments. Proceedings of the National Academy of Sciences, 102(33), 11657â11662.

Authors 2

Meredith M. Murr (first)
Daniel E. Morse (additional)

References 51 Referenced 131

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Dates

Type	When
Created	20 years ago (Aug. 9, 2005, 10:53 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 10:25 a.m.)
Indexed	2 months, 3 weeks ago (June 3, 2025, 9:28 a.m.)
Issued	20 years ago (Aug. 9, 2005)
Published	20 years ago (Aug. 9, 2005)
Published Online	20 years ago (Aug. 9, 2005)
Published Print	20 years ago (Aug. 16, 2005)

Funders 0

None

BibTeX

@article{Murr_2005, title={Fractal intermediates in the self-assembly of silicatein filaments}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0503968102}, DOI={10.1073/pnas.0503968102}, number={33}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Murr, Meredith M. and Morse, Daniel E.}, year={2005}, month=aug, pages={11657–11662} }

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