DOI: 10.1073/pnas.0503893102. Structure of dimeric mitochondrial ATP synthase: Novel F <sub>0</sub> bridging features and the structural basis of mitochondrial cristae biogenesis

Structure of dimeric mitochondrial ATP synthase: Novel F 0 bridging features and the structural basis of mitochondrial cristae biogenesis

10.1073/pnas.0503893102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The F 1 F 0 –ATP synthase exists as a dimer in mitochondria, where it is essential for the biogenesis of the inner membrane cristae. How two ATP synthase complexes dimerize to promote cristae formation is unknown. Here we resolved the structure of the dimeric F 1 F 0 ATP synthase complex isolated from bovine heart mitochondria by transmission electron microscopy. The structure of the ATP synthase dimer has an overall conic appearance that is consistent with the proposed role of the dimeric enzyme in mitochondrial cristae biogenesis. The ATP synthase dimer interface is formed by contacts on both the F 0 and F 1 domains. A cross-bridging protein density was resolved which connects the two F 0 domains on the intermembrane space side of the membrane. On the matrix side of the complex, the two F 1 moieties are connected by a protein bridge, which is attributable to the IF 1 inhibitor protein.

Bibliography

Minauro-Sanmiguel, F., Wilkens, S., & GarcÃa, J. J. (2005). Structure of dimeric mitochondrial ATP synthase: Novel F 0 bridging features and the structural basis of mitochondrial cristae biogenesis. Proceedings of the National Academy of Sciences, 102(35), 12356â12358.

Authors 3

Fernando Minauro-Sanmiguel (first)
Stephan Wilkens (additional)
José J. García (additional)

References 23 Referenced 168

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Dates

Type	When
Created	20 years ago (Aug. 16, 2005, 10:29 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 2:24 p.m.)
Indexed	1 month, 2 weeks ago (July 7, 2025, 2:46 a.m.)
Issued	20 years ago (Aug. 16, 2005)
Published	20 years ago (Aug. 16, 2005)
Published Online	20 years ago (Aug. 16, 2005)
Published Print	19 years, 11 months ago (Aug. 30, 2005)

Funders 0

None

BibTeX

@article{Minauro_Sanmiguel_2005, title={Structure of dimeric mitochondrial ATP synthase: Novel F 0 bridging features and the structural basis of mitochondrial cristae biogenesis}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0503893102}, DOI={10.1073/pnas.0503893102}, number={35}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Minauro-Sanmiguel, Fernando and Wilkens, Stephan and García, José J.}, year={2005}, month=aug, pages={12356–12358} }

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