DOI: 10.1073/pnas.0503654102. Bacterial genome size reduction by experimental evolution

Bacterial genome size reduction by experimental evolution

10.1073/pnas.0503654102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Bacterial evolution toward endosymbiosis with eukaryotic cells is associated with extensive bacterial genome reduction and loss of metabolic and regulatory capabilities. Here we examined the rate and process of genome reduction in the bacterium Salmonella enterica by a serial passage experimental evolution procedure. The initial rate of DNA loss was estimated to be 0.05 bp per chromosome per generation for a WT bacterium and ≈50-fold higher for a mutS mutant defective in methyl-directed DNA mismatch repair. The endpoints were identified for seven chromosomal deletions isolated during serial passage and in two separate genetic selections. Deletions ranged in size from 1 to 202 kb, and most of them were not associated with DNA repeats, indicating that they were formed via RecA-independent recombination events. These results suggest that extensive genome reduction can occur on a short evolutionary time scale and that RecA-dependent homologous recombination only plays a limited role in this process of jettisoning superfluous DNA.

Bibliography

Nilsson, A. I., Koskiniemi, S., Eriksson, S., Kugelberg, E., Hinton, J. C. D., & Andersson, D. I. (2005). Bacterial genome size reduction by experimental evolution. Proceedings of the National Academy of Sciences, 102(34), 12112â12116.

Authors 6

A. I. Nilsson (first)
S. Koskiniemi (additional)
S. Eriksson (additional)
E. Kugelberg (additional)
J. C. D. Hinton (additional)
D. I. Andersson (additional)

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Dates

Type	When
Created	20 years ago (Aug. 12, 2005, 8:26 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 2:19 p.m.)
Indexed	1 week, 2 days ago (Aug. 24, 2025, 6:57 p.m.)
Issued	20 years ago (Aug. 12, 2005)
Published	20 years ago (Aug. 12, 2005)
Published Online	20 years ago (Aug. 12, 2005)
Published Print	20 years ago (Aug. 23, 2005)

Funders 0

None

BibTeX

@article{Nilsson_2005, title={Bacterial genome size reduction by experimental evolution}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0503654102}, DOI={10.1073/pnas.0503654102}, number={34}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Nilsson, A. I. and Koskiniemi, S. and Eriksson, S. and Kugelberg, E. and Hinton, J. C. D. and Andersson, D. I.}, year={2005}, month=aug, pages={12112–12116} }

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