DOI: 10.1073/pnas.0503596102. Robust hepatitis C virus infection<i>in vitro</i>

Robust hepatitis C virus infectionin vitro

10.1073/pnas.0503596102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The absence of a robust cell culture model of hepatitis C virus (HCV) infection has severely limited analysis of the HCV life cycle and the development of effective antivirals and vaccines. Here we report the establishment of a simple yet robust HCV cell culture infection system based on the HCV JFH-1 molecular clone and Huh-7-derived cell lines that allows the production of virus that can be efficiently propagated in tissue culture. This system provides a powerful tool for the analysis of host-virus interactions that should facilitate the discovery of antiviral drugs and vaccines for this important human pathogen.

Bibliography

Zhong, J., Gastaminza, P., Cheng, G., Kapadia, S., Kato, T., Burton, D. R., Wieland, S. F., Uprichard, S. L., Wakita, T., & Chisari, F. V. (2005). Robust hepatitis C virus infectionin vitro. Proceedings of the National Academy of Sciences, 102(26), 9294â9299.

Authors 10

Jin Zhong (first)
Pablo Gastaminza (additional)
Guofeng Cheng (additional)
Sharookh Kapadia (additional)
Takanobu Kato (additional)
Dennis R. Burton (additional)
Stefan F. Wieland (additional)
Susan L. Uprichard (additional)
Takaji Wakita (additional)
Francis V. Chisari (additional)

References 51 Referenced 1,488

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Dates

Type	When
Created	20 years, 2 months ago (June 6, 2005, 9:58 p.m.)
Deposited	7 months, 4 weeks ago (Jan. 1, 2025, 9:41 a.m.)
Indexed	16 hours, 44 minutes ago (Aug. 29, 2025, 6:31 a.m.)
Issued	20 years, 2 months ago (June 6, 2005)
Published	20 years, 2 months ago (June 6, 2005)
Published Online	20 years, 2 months ago (June 6, 2005)
Published Print	20 years, 2 months ago (June 28, 2005)

Funders 0

None

BibTeX

@article{Zhong_2005, title={Robust hepatitis C virus infectionin vitro}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0503596102}, DOI={10.1073/pnas.0503596102}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Zhong, Jin and Gastaminza, Pablo and Cheng, Guofeng and Kapadia, Sharookh and Kato, Takanobu and Burton, Dennis R. and Wieland, Stefan F. and Uprichard, Susan L. and Wakita, Takaji and Chisari, Francis V.}, year={2005}, month=jun, pages={9294–9299} }

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