DOI: 10.1073/pnas.0502718103. Microfibrillar structure of type I collagen <i>in situ</i>

Microfibrillar structure of type I collagen in situ

10.1073/pnas.0502718103

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The fibrous collagens are ubiquitous in animals and form the structural basis of all mammalian connective tissues, including those of the heart, vasculature, skin, cornea, bones, and tendons. However, in comparison with what is known of their production, turnover and physiological structure, very little is understood regarding the three-dimensional arrangement of collagen molecules in naturally occurring fibrils. This knowledge may provide insight into key biological processes such as fibrillo-genesis and tissue remodeling and into diseases such as heart disease and cancer. Here we present a crystallographic determination of the collagen type I supermolecular structure, where the molecular conformation of each collagen segment found within the naturally occurring crystallographic unit cell has been defined (P1, a ≈ 40.0 Å, b ≈ 27.0 Å, c ≈ 678 Å, α ≈ 89.2°, β ≈ 94.6°, γ ≈ 105.6°; reflections: 414, overlapping, 232, and nonoverlapping, 182; resolution, 5.16 Å axial and 11.1 Å equatorial). This structure shows that the molecular packing topology of the collagen molecule is such that packing neighbors are arranged to form a supertwisted (discontinuous) right-handed microfibril that interdigitates with neighboring microfibrils. This interdigitation establishes the crystallographic superlattice, which is formed of quasihexagonally packed collagen molecules. In addition, the molecular packing structure of collagen shown here provides information concerning the potential modes of action of two prominent molecules involved in human health and disease: decorin and the Matrix Metallo-Proteinase (MMP) collagenase.

Bibliography

Orgel, J. P. R. O., Irving, T. C., Miller, A., & Wess, T. J. (2006). Microfibrillar structure of type I collagen in situ. Proceedings of the National Academy of Sciences, 103(24), 9001â9005.

Authors 4

Joseph P. R. O. Orgel (first)
Thomas C. Irving (additional)
Andrew Miller (additional)
Tim J. Wess (additional)

References 42 Referenced 832

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Dates

Type	When
Created	19 years, 3 months ago (June 2, 2006, 8:47 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 1:51 p.m.)
Indexed	18 minutes ago (Sept. 3, 2025, 5:48 p.m.)
Issued	19 years, 2 months ago (June 13, 2006)
Published	19 years, 2 months ago (June 13, 2006)
Published Online	19 years, 2 months ago (June 13, 2006)
Published Print	19 years, 2 months ago (June 13, 2006)

Funders 0

None

BibTeX

@article{Orgel_2006, title={Microfibrillar structure of type I collagen in situ}, volume={103}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0502718103}, DOI={10.1073/pnas.0502718103}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Orgel, Joseph P. R. O. and Irving, Thomas C. and Miller, Andrew and Wess, Tim J.}, year={2006}, month=jun, pages={9001–9005} }

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