DOI: 10.1073/pnas.0502040102. Evasion of Toll-like receptor 5 by flagellated bacteria

Evasion of Toll-like receptor 5 by flagellated bacteria

10.1073/pnas.0502040102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Toll-like receptor 5 (TLR5) recognizes an evolutionarily conserved site on bacterial flagellin that is required for flagellar filament assembly and motility. The α and ε Proteobacteria , including the important human pathogens Campylobacter jejuni , Helicobacter pylori , and Bartonella bacilliformis , require flagellar motility to efficiently infect mammalian hosts. In this study, we demonstrate that these bacteria make flagellin molecules that are not recognized by TLR5. We map the site responsible for TLR5 evasion to amino acids 89-96 of the N-terminal D1 domain, which is centrally positioned within the previously defined TLR5 recognition site. Salmonella flagellin is strongly recognized by TLR5, but mutating residues 89-96 to the corresponding H. pylori flaA sequence abolishes TLR5 recognition and also destroys bacterial motility. To preserve bacterial motility, α and ε Proteobacteria possess compensatory amino acid changes in other regions of the flagellin molecule, and we engineer a mutant form of Salmonella flagellin that evades TLR5 but retains motility. These results suggest that TLR5 evasion is critical for the survival of this subset of bacteria at mucosal sites in animals and raise the intriguing possibility that flagellin receptors provided the selective force to drive the evolution of these unique subclasses of bacterial flagellins.

Bibliography

Andersen-Nissen, E., Smith, K. D., Strobe, K. L., Barrett, S. L. R., Cookson, B. T., Logan, S. M., & Aderem, A. (2005). Evasion of Toll-like receptor 5 by flagellated bacteria. Proceedings of the National Academy of Sciences, 102(26), 9247â9252.

Authors 7

Erica Andersen-Nissen (first)
Kelly D. Smith (additional)
Katie L. Strobe (additional)
Sara L. Rassoulian Barrett (additional)
Brad T. Cookson (additional)
Susan M. Logan (additional)
Alan Aderem (additional)

References 46 Referenced 538

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Dates

Type	When
Created	20 years, 2 months ago (June 15, 2005, 6:18 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 10:15 a.m.)
Indexed	1 week, 3 days ago (Aug. 12, 2025, 5:51 p.m.)
Issued	20 years, 2 months ago (June 13, 2005)
Published	20 years, 2 months ago (June 13, 2005)
Published Online	20 years, 2 months ago (June 13, 2005)
Published Print	20 years, 1 month ago (June 28, 2005)

Funders 0

None

BibTeX

@article{Andersen_Nissen_2005, title={Evasion of Toll-like receptor 5 by flagellated bacteria}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0502040102}, DOI={10.1073/pnas.0502040102}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Andersen-Nissen, Erica and Smith, Kelly D. and Strobe, Katie L. and Barrett, Sara L. Rassoulian and Cookson, Brad T. and Logan, Susan M. and Aderem, Alan}, year={2005}, month=jun, pages={9247–9252} }

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