DOI: 10.1073/pnas.0501581102. Dissecting the mechanical unfolding of ubiquitin

Dissecting the mechanical unfolding of ubiquitin

10.1073/pnas.0501581102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The unfolding behavior of ubiquitin under the influence of a stretching force recently was investigated experimentally by single-molecule constant-force methods. Many observed unfolding traces had a simple two-state character, whereas others showed clear evidence of intermediate states. Here, we use Monte Carlo simulations to investigate the force-induced unfolding of ubiquitin at the atomic level. In agreement with experimental data, we find that the unfolding process can occur either in a single step or through intermediate states. In addition to this randomness, we find that many quantities, such as the frequency of occurrence of intermediates, show a clear systematic dependence on the strength of the applied force. Despite this diversity, one common feature can be identified in the simulated unfolding events, which is the order in which the secondary-structure elements break. This order is the same in two- and three-state events and at the different forces studied. The observed order remains to be verified experimentally but appears physically reasonable.

Bibliography

IrbÃ¤ck, A., Mitternacht, S., & Mohanty, S. (2005). Dissecting the mechanical unfolding of ubiquitin. Proceedings of the National Academy of Sciences, 102(38), 13427â13432.

Authors 3

Anders Irbäck (first)
Simon Mitternacht (additional)
Sandipan Mohanty (additional)

References 38 Referenced 67

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Dates

Type	When
Created	19 years, 11 months ago (Sept. 7, 2005, 8:25 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 2:16 p.m.)
Indexed	1 year, 1 month ago (Aug. 6, 2024, 10:55 a.m.)
Issued	19 years, 11 months ago (Sept. 7, 2005)
Published	19 years, 11 months ago (Sept. 7, 2005)
Published Online	19 years, 11 months ago (Sept. 7, 2005)
Published Print	19 years, 11 months ago (Sept. 20, 2005)

Funders 0

None

BibTeX

@article{Irb_ck_2005, title={Dissecting the mechanical unfolding of ubiquitin}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0501581102}, DOI={10.1073/pnas.0501581102}, number={38}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Irbäck, Anders and Mitternacht, Simon and Mohanty, Sandipan}, year={2005}, month=sep, pages={13427–13432} }

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